Zinc protects against arsenic-induced apoptosis in a neuronal cell line, measured by DEVD-caspase activity

Austin Milton, Peter David Zalewski, Ranjit Nihal Ratnaike

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Acute and chronic arsenic exposure results in toxicity in humans and causes many neurological and other manifestations. For the first time the present study reports that zinc decreases arsenic-induced apoptosis and also confirms a single report of apoptosis induced by arsenic in a neuronal cell line. Apoptosis measured by DEVD-caspase activity peaked between 10 μM and 20 μM of arsenic trioxide. Higher concentrations of arsenic up to 40 μM caused increasing cell death with diminishing DEVD-caspase activity. The beneficial effect of zinc was proportional to its concentration with a significant decrease in arsenic-induced DEVD-caspase activity at 50 μM and 75 μM zinc (P < 0.05). This finding may be of therapeutic benefit in people suffering from chronic exposure to arsenic from natural sources, a global problem especially relevant to millions of people on the Indian subcontinent.

Original languageEnglish
Pages (from-to)707-713
Number of pages7
JournalBioMetals
Volume17
Issue number6
DOIs
Publication statusPublished - 1 Dec 2004
Externally publishedYes

Keywords

  • 2.3D mouse neuroepithelial cells
  • Arsenic trioxide
  • DEVD-caspase
  • Zinc sulfate

ASJC Scopus subject areas

  • Biomaterials
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Metals and Alloys

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