VCAM-1 is not involved in LPAM-1 (α4β(p)/α4β7) mediated binding of lymphoma cells to high endothelial venules of mucosa-associated lymph nodes

M. Hahne, M. Lenter, U. Jager, Sandra Isenmann, D. Vestweber

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27 Citations (Scopus)

Abstract

The migration of lymphocytes from the blood into various lymphoid organs (lymphocyte homing) occurs in specialized high endothelial venules (HEV). Homing of lymphocytes into mucosa-associated lymph nodes in the mouse is mediated at least in part by the lymphocyte homing receptor LPAM-1, an integrin which has been identified as the heterodimer α4β(p) (α4β7 in humans). The HEV-ligand for this integrin in the homing process has not yet been identified. VCAM-1, a cytokine-inducible, endothelial adhesion molecule for lymphocytes and monocytes, was recently demonstrated in vitro to be a ligand for the α4β(p) integrin. We have tested whether VCAM-1 could be a candidate for an α4β(p) ligand on HEV. Two findings strongly argue against this possibility: First, an anti-VCAM-1 monoclonal antibody (mAb) failed to block the binding of the mouse T-lymphona TK-1 to HEV in cryostat sections of mesenteric lymph nodes, even though this antibody blocked α4β(p) mediated binding of TK-1 cells to VCAM-1 on TNF-α-activated mouse endothelioma cells. Second, expression of VCAM-1 on lymph node endothelium was undetectably low as tested by immunofluorescence staining of mouse tissue sections using four different mAbs against mouse VCAM-1, three of which are described in this report. Surprisingly, expression of VCAM-1 on lymph node endothelium was not found even in mice which had been treated systemically with high doses of TNF-α or LPS, although VCAM-1 expression was strongly induced on endothelia of all other organs that were analyzed. This demonstrates that lymph node endothelium differs from endothelia in other tissues in its regulation of the expression of VCAM-1.

Original languageEnglish
Pages (from-to)290-298
Number of pages9
JournalEuropean Journal of Cell Biology
Volume61
Issue number2
Publication statusPublished or Issued - 1 Jan 1993

Keywords

  • Cell adhesion
  • Cytokines
  • Endothelial cells
  • Lymphocyte homing

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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