Uveal mast cells are not required for rodent uveitis

Justine R. Smith, Prue H. Hart, Douglas J. Coster, Keryn Williams

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Uveal mast cells have previously been considered to be vital mediators of experimental uveitis. We extended the study of these cells to experimental melanin-induced uveitis (EMIU), a recently described clinically relevant model, and re-examined their role in endotoxin-induced uveitis (EIU) using genetically mast cell-depleted mice on a single background. EMIU was induced in Fischer 344 rats by immunisation with bovine ocular melanin (250 μg). Animals were killed immediately, and on days 1 and 3 of clinical disease. Numbers of uveal mast cells and the percentage of degranulated cells were counted in whole-mount preparations. There was no significant change in either measure across the selected time points. To induce EIU, normal and mast cell-depleted DBA/2 mice were injected with Escherichia coli lipopolysaccharide (400 μg). Cells infiltrating the eye 24 h after injection were quantified in 5 μm ocular cross-sections. Disease was not significantly reduced in the mast cell-depleted mutants. We conclude that uveal mast cells are not required for the development of EMIU or, in contrast to earlier work, EIU.

LanguageEnglish
Pages388-393
Number of pages6
JournalOphthalmic Research
Volume30
Issue number6
DOIs
Publication statusPublished - 1 Nov 1998
Externally publishedYes

Keywords

  • Endotoxin-induced uveitis
  • Experimental melanin-induced uveitis
  • Mouse
  • Rat
  • Uveal mast cell

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Smith, Justine R. ; Hart, Prue H. ; Coster, Douglas J. ; Williams, Keryn. / Uveal mast cells are not required for rodent uveitis. In: Ophthalmic Research. 1998 ; Vol. 30, No. 6. pp. 388-393.
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Uveal mast cells are not required for rodent uveitis. / Smith, Justine R.; Hart, Prue H.; Coster, Douglas J.; Williams, Keryn.

In: Ophthalmic Research, Vol. 30, No. 6, 01.11.1998, p. 388-393.

Research output: Contribution to journalArticle

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