Abstract
Uveal mast cells have previously been considered to be vital mediators of experimental uveitis. We extended the study of these cells to experimental melanin-induced uveitis (EMIU), a recently described clinically relevant model, and re-examined their role in endotoxin-induced uveitis (EIU) using genetically mast cell-depleted mice on a single background. EMIU was induced in Fischer 344 rats by immunisation with bovine ocular melanin (250 μg). Animals were killed immediately, and on days 1 and 3 of clinical disease. Numbers of uveal mast cells and the percentage of degranulated cells were counted in whole-mount preparations. There was no significant change in either measure across the selected time points. To induce EIU, normal and mast cell-depleted DBA/2 mice were injected with Escherichia coli lipopolysaccharide (400 μg). Cells infiltrating the eye 24 h after injection were quantified in 5 μm ocular cross-sections. Disease was not significantly reduced in the mast cell-depleted mutants. We conclude that uveal mast cells are not required for the development of EMIU or, in contrast to earlier work, EIU.
| Language | English |
|---|---|
| Pages | 388-393 |
| Number of pages | 6 |
| Journal | Ophthalmic Research |
| Volume | 30 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1 Nov 1998 |
| Externally published | Yes |
Keywords
- Endotoxin-induced uveitis
- Experimental melanin-induced uveitis
- Mouse
- Rat
- Uveal mast cell
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
- Cellular and Molecular Neuroscience
Cite this
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Uveal mast cells are not required for rodent uveitis. / Smith, Justine R.; Hart, Prue H.; Coster, Douglas J.; Williams, Keryn.
In: Ophthalmic Research, Vol. 30, No. 6, 01.11.1998, p. 388-393.Research output: Contribution to journal › Article
TY - JOUR
T1 - Uveal mast cells are not required for rodent uveitis
AU - Smith, Justine R.
AU - Hart, Prue H.
AU - Coster, Douglas J.
AU - Williams, Keryn
PY - 1998/11/1
Y1 - 1998/11/1
N2 - Uveal mast cells have previously been considered to be vital mediators of experimental uveitis. We extended the study of these cells to experimental melanin-induced uveitis (EMIU), a recently described clinically relevant model, and re-examined their role in endotoxin-induced uveitis (EIU) using genetically mast cell-depleted mice on a single background. EMIU was induced in Fischer 344 rats by immunisation with bovine ocular melanin (250 μg). Animals were killed immediately, and on days 1 and 3 of clinical disease. Numbers of uveal mast cells and the percentage of degranulated cells were counted in whole-mount preparations. There was no significant change in either measure across the selected time points. To induce EIU, normal and mast cell-depleted DBA/2 mice were injected with Escherichia coli lipopolysaccharide (400 μg). Cells infiltrating the eye 24 h after injection were quantified in 5 μm ocular cross-sections. Disease was not significantly reduced in the mast cell-depleted mutants. We conclude that uveal mast cells are not required for the development of EMIU or, in contrast to earlier work, EIU.
AB - Uveal mast cells have previously been considered to be vital mediators of experimental uveitis. We extended the study of these cells to experimental melanin-induced uveitis (EMIU), a recently described clinically relevant model, and re-examined their role in endotoxin-induced uveitis (EIU) using genetically mast cell-depleted mice on a single background. EMIU was induced in Fischer 344 rats by immunisation with bovine ocular melanin (250 μg). Animals were killed immediately, and on days 1 and 3 of clinical disease. Numbers of uveal mast cells and the percentage of degranulated cells were counted in whole-mount preparations. There was no significant change in either measure across the selected time points. To induce EIU, normal and mast cell-depleted DBA/2 mice were injected with Escherichia coli lipopolysaccharide (400 μg). Cells infiltrating the eye 24 h after injection were quantified in 5 μm ocular cross-sections. Disease was not significantly reduced in the mast cell-depleted mutants. We conclude that uveal mast cells are not required for the development of EMIU or, in contrast to earlier work, EIU.
KW - Endotoxin-induced uveitis
KW - Experimental melanin-induced uveitis
KW - Mouse
KW - Rat
KW - Uveal mast cell
UR - http://www.scopus.com/inward/record.url?scp=0031718030&partnerID=8YFLogxK
U2 - 10.1159/000055500
DO - 10.1159/000055500
M3 - Article
VL - 30
SP - 388
EP - 393
JO - Ophthalmic Research
T2 - Ophthalmic Research
JF - Ophthalmic Research
SN - 0030-3747
IS - 6
ER -