Abstract
Uveal mast cells have previously been considered to be vital mediators of experimental uveitis. We extended the study of these cells to experimental melanin-induced uveitis (EMIU), a recently described clinically relevant model, and re-examined their role in endotoxin-induced uveitis (EIU) using genetically mast cell-depleted mice on a single background. EMIU was induced in Fischer 344 rats by immunisation with bovine ocular melanin (250 μg). Animals were killed immediately, and on days 1 and 3 of clinical disease. Numbers of uveal mast cells and the percentage of degranulated cells were counted in whole-mount preparations. There was no significant change in either measure across the selected time points. To induce EIU, normal and mast cell-depleted DBA/2 mice were injected with Escherichia coli lipopolysaccharide (400 μg). Cells infiltrating the eye 24 h after injection were quantified in 5 μm ocular cross-sections. Disease was not significantly reduced in the mast cell-depleted mutants. We conclude that uveal mast cells are not required for the development of EMIU or, in contrast to earlier work, EIU.
Original language | English |
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Pages (from-to) | 388-393 |
Number of pages | 6 |
Journal | Ophthalmic Research |
Volume | 30 |
Issue number | 6 |
DOIs | |
Publication status | Published - 1 Nov 1998 |
Externally published | Yes |
Keywords
- Endotoxin-induced uveitis
- Experimental melanin-induced uveitis
- Mouse
- Rat
- Uveal mast cell
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
- Cellular and Molecular Neuroscience