Use of laser capture microdissection to detect integrated HIV-1 DNA in macrophages and astrocytes from autopsy brain tissues

Melissa J. Churchill, Paul R. Gorry, Daniel Cowley, Luxshimi Lal, Secondo Sonza, Damian F.J. Purcell, Katherine A. Thompson, Dana Gabuzda, Justin C. McArthur, Carlos A. Pardo, Steven Wesselingh

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

The importance of astrocytes as a reservoir of human immunodeficiency virus type 1 (HIV-1) in the brain remains elusive. By combining immunohistochemistry, laser capture microdissection, and triple-nested Alu-PCR, we demonstrate integrated HIV-1 in astrocytes and macrophages isolated directly from autopsy brain tissues of HIV-1-infected subjects. The ability of HIV-1 to integrate in terminally differentiated astrocytes suggests a permanent reservoir of provirus in brain that will impact the development and likely success of strategies aimed at eradicating HIV-1.

LanguageEnglish
Pages146-152
Number of pages7
JournalJournal of NeuroVirology
Volume12
Issue number2
DOIs
Publication statusPublished - 1 Apr 2006
Externally publishedYes

Keywords

  • Astrocyte
  • HIV-1
  • HIVD
  • HIVE
  • Macrophage
  • Provirus

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Virology

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