Transcriptional and epigenetic mechanisms of cellular reprogramming to induced pluripotency

Mark Van Den Hurk, Gunter Kenis, Cedric Bardy, Daniel L. Van Den Hove, Fred H. Gage, Harry W. Steinbusch, Bart P. Rutten

Research output: Contribution to journalReview articlepeer-review

12 Citations (Scopus)

Abstract

Enforced ectopic expression of a cocktail of pluripotency-associated genes such as Oct4, Sox2, Klf4 and c-Myc can reprogram somatic cells into induced pluripotent stem cells (iPSCs). The remarkable proliferation ability of iPSCs and their aptitude to redifferentiate into any cell lineage makes these cells a promising tool for generating a variety of human tissue in vitro. Yet, pluripotency induction is an inefficient process, as cells undergoing reprogramming need to overcome developmentally imposed epigenetic barriers. Recent work has shed new light on the molecular mechanisms that drive the reprogramming of somatic cells to iPSCs. Here, we present current knowledge on the transcriptional and epigenetic regulation of pluripotency induction and discuss how variability in epigenetic states impacts iPSCs' inherent biological properties.

Original languageEnglish
Pages (from-to)1131-1149
Number of pages19
JournalEpigenomics
Volume8
Issue number8
DOIs
Publication statusPublished or Issued - Aug 2016
Externally publishedYes

Keywords

  • DNA methylation
  • chromatin
  • epigenetics
  • histone modifications
  • iPSC
  • induced pluripotent stem cell
  • pluripotency
  • reprogramming

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

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