Toxocara canis larval excretory/secretory proteins impair eosinophil-dependent resistance of mice to Nippostrongylus brasiliensis

P. R. Giacomin, M. Cava, D. J. Tumes, A. D. Gauld, D. R. Iddawela, S. R. Mccoll, J. C. Parsons, D. L. Gordon, L. A. Dent

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Survival of parasitic helminths within a host requires immune evasion and excretorylsecretory (ES) proteins may contribute to this process. Eosinophils are important effector cells in immunity of mice to the nematode Nippostrongylus brasiliensis and eosinophilic interleukin-5 transgenic (IL-5 Tg) mice are highly resistant to the earliest stages of primary infections. In contrast, Toxocara canis is largely resistant to eosinophils, with viable larvae encysted in tissues often surrounded by these and other leucocytes. The aim of this study was to investigate whether T. canis ES (TES) proteins inhibit eosinophil-dependent resistance to N. brasiliensis. Mouse serum pre-treated with TES had reduced capacity to mediate the adherence of leucocytes to N. brasiliensis infective-stage larvae (L3) and this correlated with reduced complement C3 deposition on the parasite. TES did not inhibit eosinophil survival or eotaxin-dependent eosinophil migration in vitro. Cellular inflammation and eosinophil degranulation in the skin in response to injection of L3 was also not impaired by TES. However, when TES was included with L3 in an inoculum given to IL-5 Tg mice, a greatly increased number of parasites migrated to the lung. This suggests that the early eosinophil-dependent resistance in these mice was suppressed, by mechanisms yet to be determined.

LanguageEnglish
Pages435-445
Number of pages11
JournalParasite Immunology
Volume30
Issue number8
DOIs
Publication statusPublished - Aug 2008

Keywords

  • Eosinophil
  • Excretorylsecretory protein
  • Immune evasion
  • Nippostrongylus brasiliensis
  • Toxocara canis

ASJC Scopus subject areas

  • Parasitology
  • Immunology

Cite this

Giacomin, P. R., Cava, M., Tumes, D. J., Gauld, A. D., Iddawela, D. R., Mccoll, S. R., ... Dent, L. A. (2008). Toxocara canis larval excretory/secretory proteins impair eosinophil-dependent resistance of mice to Nippostrongylus brasiliensis. Parasite Immunology, 30(8), 435-445. https://doi.org/10.1111/j.1365-3024.2008.01040.x
Giacomin, P. R. ; Cava, M. ; Tumes, D. J. ; Gauld, A. D. ; Iddawela, D. R. ; Mccoll, S. R. ; Parsons, J. C. ; Gordon, D. L. ; Dent, L. A. / Toxocara canis larval excretory/secretory proteins impair eosinophil-dependent resistance of mice to Nippostrongylus brasiliensis. In: Parasite Immunology. 2008 ; Vol. 30, No. 8. pp. 435-445.
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abstract = "Survival of parasitic helminths within a host requires immune evasion and excretorylsecretory (ES) proteins may contribute to this process. Eosinophils are important effector cells in immunity of mice to the nematode Nippostrongylus brasiliensis and eosinophilic interleukin-5 transgenic (IL-5 Tg) mice are highly resistant to the earliest stages of primary infections. In contrast, Toxocara canis is largely resistant to eosinophils, with viable larvae encysted in tissues often surrounded by these and other leucocytes. The aim of this study was to investigate whether T. canis ES (TES) proteins inhibit eosinophil-dependent resistance to N. brasiliensis. Mouse serum pre-treated with TES had reduced capacity to mediate the adherence of leucocytes to N. brasiliensis infective-stage larvae (L3) and this correlated with reduced complement C3 deposition on the parasite. TES did not inhibit eosinophil survival or eotaxin-dependent eosinophil migration in vitro. Cellular inflammation and eosinophil degranulation in the skin in response to injection of L3 was also not impaired by TES. However, when TES was included with L3 in an inoculum given to IL-5 Tg mice, a greatly increased number of parasites migrated to the lung. This suggests that the early eosinophil-dependent resistance in these mice was suppressed, by mechanisms yet to be determined.",
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Giacomin, PR, Cava, M, Tumes, DJ, Gauld, AD, Iddawela, DR, Mccoll, SR, Parsons, JC, Gordon, DL & Dent, LA 2008, 'Toxocara canis larval excretory/secretory proteins impair eosinophil-dependent resistance of mice to Nippostrongylus brasiliensis', Parasite Immunology, vol. 30, no. 8, pp. 435-445. https://doi.org/10.1111/j.1365-3024.2008.01040.x

Toxocara canis larval excretory/secretory proteins impair eosinophil-dependent resistance of mice to Nippostrongylus brasiliensis. / Giacomin, P. R.; Cava, M.; Tumes, D. J.; Gauld, A. D.; Iddawela, D. R.; Mccoll, S. R.; Parsons, J. C.; Gordon, D. L.; Dent, L. A.

In: Parasite Immunology, Vol. 30, No. 8, 08.2008, p. 435-445.

Research output: Contribution to journalArticle

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T1 - Toxocara canis larval excretory/secretory proteins impair eosinophil-dependent resistance of mice to Nippostrongylus brasiliensis

AU - Giacomin, P. R.

AU - Cava, M.

AU - Tumes, D. J.

AU - Gauld, A. D.

AU - Iddawela, D. R.

AU - Mccoll, S. R.

AU - Parsons, J. C.

AU - Gordon, D. L.

AU - Dent, L. A.

PY - 2008/8

Y1 - 2008/8

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KW - Excretorylsecretory protein

KW - Immune evasion

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