Towards the commercialization of Botryococcus braunii for triterpenoid production

Khalid A. Al-Hothaly, Eric M. Adetutu, Brian H. May, Mohamed Taha, Andrew S. Ball

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Botryococcus braunii can accumulate unusually high levels of triterpenoid hydrocarbons making it a potential source of high value chemicals. However, its commercial application is hampered by its slow growth and lack of large-scale studies of triterpenoid hydrocarbon production. This study investigated hydrocarbon production in two race B of B. braunii strains, Overjuyo-3 and Kossou-4, at 25 °C in 500 L open tanks under artificial lighting in modified BG11 medium over 60 days. Maximum growth was reached by 40 days with Overjuyo-3 producing more biomass (3.05 g L−1) than Kossou-4 (2.55 g L−1). However, Kossou-4 produced more oil (0.75 g L−1) and triterpenoid hydrocarbons (C30–C34; 50 % of oil weight) compared to 0.63 g L−1 of oil in Overjuyo-3 with triterpenoid hydrocarbons making up 29 % of oil weight. This research demonstrates for the first time that large-scale production of high value triterpenoid hydrocarbon for commercial application is feasible with Kossou-4 strain.

LanguageEnglish
Pages1415-1418
Number of pages4
JournalJournal of Industrial Microbiology and Biotechnology
Volume42
Issue number10
DOIs
Publication statusPublished - 26 Oct 2015
Externally publishedYes

Keywords

  • Biomass
  • Botryococcus braunii
  • Large scale
  • Race B
  • Triterpenoid hydrocarbon

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Al-Hothaly, Khalid A. ; Adetutu, Eric M. ; May, Brian H. ; Taha, Mohamed ; Ball, Andrew S. / Towards the commercialization of Botryococcus braunii for triterpenoid production. In: Journal of Industrial Microbiology and Biotechnology. 2015 ; Vol. 42, No. 10. pp. 1415-1418.
@article{58913b32888b4a20b7b781644e2ca835,
title = "Towards the commercialization of Botryococcus braunii for triterpenoid production",
abstract = "Botryococcus braunii can accumulate unusually high levels of triterpenoid hydrocarbons making it a potential source of high value chemicals. However, its commercial application is hampered by its slow growth and lack of large-scale studies of triterpenoid hydrocarbon production. This study investigated hydrocarbon production in two race B of B. braunii strains, Overjuyo-3 and Kossou-4, at 25 °C in 500 L open tanks under artificial lighting in modified BG11 medium over 60 days. Maximum growth was reached by 40 days with Overjuyo-3 producing more biomass (3.05 g L−1) than Kossou-4 (2.55 g L−1). However, Kossou-4 produced more oil (0.75 g L−1) and triterpenoid hydrocarbons (C30–C34; 50 {\%} of oil weight) compared to 0.63 g L−1 of oil in Overjuyo-3 with triterpenoid hydrocarbons making up 29 {\%} of oil weight. This research demonstrates for the first time that large-scale production of high value triterpenoid hydrocarbon for commercial application is feasible with Kossou-4 strain.",
keywords = "Biomass, Botryococcus braunii, Large scale, Race B, Triterpenoid hydrocarbon",
author = "Al-Hothaly, {Khalid A.} and Adetutu, {Eric M.} and May, {Brian H.} and Mohamed Taha and Ball, {Andrew S.}",
year = "2015",
month = "10",
day = "26",
doi = "10.1007/s10295-015-1658-x",
language = "English",
volume = "42",
pages = "1415--1418",
journal = "Journal of Industrial Microbiology and Biotechnology",
issn = "1367-5435",
publisher = "Springer Verlag",
number = "10",

}

Towards the commercialization of Botryococcus braunii for triterpenoid production. / Al-Hothaly, Khalid A.; Adetutu, Eric M.; May, Brian H.; Taha, Mohamed; Ball, Andrew S.

In: Journal of Industrial Microbiology and Biotechnology, Vol. 42, No. 10, 26.10.2015, p. 1415-1418.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Towards the commercialization of Botryococcus braunii for triterpenoid production

AU - Al-Hothaly, Khalid A.

AU - Adetutu, Eric M.

AU - May, Brian H.

AU - Taha, Mohamed

AU - Ball, Andrew S.

PY - 2015/10/26

Y1 - 2015/10/26

N2 - Botryococcus braunii can accumulate unusually high levels of triterpenoid hydrocarbons making it a potential source of high value chemicals. However, its commercial application is hampered by its slow growth and lack of large-scale studies of triterpenoid hydrocarbon production. This study investigated hydrocarbon production in two race B of B. braunii strains, Overjuyo-3 and Kossou-4, at 25 °C in 500 L open tanks under artificial lighting in modified BG11 medium over 60 days. Maximum growth was reached by 40 days with Overjuyo-3 producing more biomass (3.05 g L−1) than Kossou-4 (2.55 g L−1). However, Kossou-4 produced more oil (0.75 g L−1) and triterpenoid hydrocarbons (C30–C34; 50 % of oil weight) compared to 0.63 g L−1 of oil in Overjuyo-3 with triterpenoid hydrocarbons making up 29 % of oil weight. This research demonstrates for the first time that large-scale production of high value triterpenoid hydrocarbon for commercial application is feasible with Kossou-4 strain.

AB - Botryococcus braunii can accumulate unusually high levels of triterpenoid hydrocarbons making it a potential source of high value chemicals. However, its commercial application is hampered by its slow growth and lack of large-scale studies of triterpenoid hydrocarbon production. This study investigated hydrocarbon production in two race B of B. braunii strains, Overjuyo-3 and Kossou-4, at 25 °C in 500 L open tanks under artificial lighting in modified BG11 medium over 60 days. Maximum growth was reached by 40 days with Overjuyo-3 producing more biomass (3.05 g L−1) than Kossou-4 (2.55 g L−1). However, Kossou-4 produced more oil (0.75 g L−1) and triterpenoid hydrocarbons (C30–C34; 50 % of oil weight) compared to 0.63 g L−1 of oil in Overjuyo-3 with triterpenoid hydrocarbons making up 29 % of oil weight. This research demonstrates for the first time that large-scale production of high value triterpenoid hydrocarbon for commercial application is feasible with Kossou-4 strain.

KW - Biomass

KW - Botryococcus braunii

KW - Large scale

KW - Race B

KW - Triterpenoid hydrocarbon

UR - http://www.scopus.com/inward/record.url?scp=84942368152&partnerID=8YFLogxK

U2 - 10.1007/s10295-015-1658-x

DO - 10.1007/s10295-015-1658-x

M3 - Article

VL - 42

SP - 1415

EP - 1418

JO - Journal of Industrial Microbiology and Biotechnology

T2 - Journal of Industrial Microbiology and Biotechnology

JF - Journal of Industrial Microbiology and Biotechnology

SN - 1367-5435

IS - 10

ER -