The use of transcriptional profiles to predict adult mosquito age under field conditions

Peter E. Cook, Leon E. Hugo, Iñaki Iturbe-Ormaetxe, Craig Williams, Stephen F. Chenoweth, Scott A. Ritchie, Peter A. Ryan, Brian H. Kay, Mark W. Blows, Scott L. O'Neill

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Age is a critical determinant of an adult female mosquito's ability to transmit a range of human pathogens. Despite its central importance, relatively few methods exist with which to accurately determine chronological age of field-caught mosquitoes. This fact is a major constraint on our ability to fully understand the relative importance of vector longevity to disease transmission in different ecological contexts. It also limits our ability to evaluate novel disease control strategies that specifically target mosquito longevity. We report the development of a transcriptional profiling approach to determine age of adult female Aedes aegypti under field conditions. We demonstrate that this approach surpasses current cuticular hydrocarbon methods for both accuracy of predicted age as well as the upper limits at which age can be reliably predicted. The method is based on genes that display age-dependent expression in a range of dipteran insects and, as such, is likely to be broadly applicable to other disease vectors.

LanguageEnglish
Pages18060-18065
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number48
DOIs
Publication statusPublished - 28 Nov 2006
Externally publishedYes

Keywords

  • Aedes aegypti
  • Age-grading
  • Cuticular hydrocarbons
  • Gene expression
  • Multivariate calibration

ASJC Scopus subject areas

  • General

Cite this

Cook, Peter E. ; Hugo, Leon E. ; Iturbe-Ormaetxe, Iñaki ; Williams, Craig ; Chenoweth, Stephen F. ; Ritchie, Scott A. ; Ryan, Peter A. ; Kay, Brian H. ; Blows, Mark W. ; O'Neill, Scott L. / The use of transcriptional profiles to predict adult mosquito age under field conditions. In: Proceedings of the National Academy of Sciences of the United States of America. 2006 ; Vol. 103, No. 48. pp. 18060-18065.
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Cook, PE, Hugo, LE, Iturbe-Ormaetxe, I, Williams, C, Chenoweth, SF, Ritchie, SA, Ryan, PA, Kay, BH, Blows, MW & O'Neill, SL 2006, 'The use of transcriptional profiles to predict adult mosquito age under field conditions', Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 48, pp. 18060-18065. https://doi.org/10.1073/pnas.0604875103

The use of transcriptional profiles to predict adult mosquito age under field conditions. / Cook, Peter E.; Hugo, Leon E.; Iturbe-Ormaetxe, Iñaki; Williams, Craig; Chenoweth, Stephen F.; Ritchie, Scott A.; Ryan, Peter A.; Kay, Brian H.; Blows, Mark W.; O'Neill, Scott L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, No. 48, 28.11.2006, p. 18060-18065.

Research output: Contribution to journalArticle

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