The taq IA and Ser311Cys polymorphisms in the dopamine D2 receptor gene and obesity

A. Southon, K. Walder, A. M. Sanigorski, Paul Zimmet, G. C. Nicholson, M. A. Kotowicz, G. Collier, Barbara Edwards

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Abstract

Dopamine D2 receptors (DRD2) in the central nervous system are involved in the regulation of feeding. It remains to be elucidated if mutations in the DRD2 gene contribute to the development of obesity. The aim of the present study was to investigate whether the Taq IA and Ser311Cys polymorphisms in the DRD2 gene are associated with obesity in Nauruan and Australian subjects. Subjects were selected based on extremes of the body mass index (BMI) distribution. Two groups of Australian women were selected. The leanest group had a mean BMI of 22.5 kg/m2 (range: 20.3-24.3) and the heaviest group had a mean of 36.1 kg/m2 (32.5-44.1). Four groups of Nauruan subjects were selected. Leanest men had a mean BMI of 33.0 kg/m2 (28.4-36.9 , heaviest men had a mean of 52.8 kg/m2 (46.5-69.2), leanest women had a mean of 34.8 kg/m2 (28.2-41.8) and heaviest women had a mean of 55.1 kg/m2 (49.3-73.8). Subjects were genotyped for the Taq IA and Ser311Cys polymorphisms using polymerase chain reaction (PCR) restriction fragment length polymorphism analysis and allelic discrimination Taqman™ PCR respectively. Leanest and heaviest groups were examined for differences in genotype frequency. Taq IA and Ser311Cys genotype frequencies did not differ significantly between leanest and heaviest Nauruan groups, or between leanest and heaviest Australians. Haplo-type frequencies of these polymorphisms did not differ between leanest and heaviest groups. The Taq IA and Ser311Cys polymorphisms in the DRD2 gene are unlikely to be common causes of obesity in these populations.

Original languageEnglish
Pages (from-to)72-76
Number of pages5
JournalDiabetes, Nutrition and Metabolism - Clinical and Experimental
Volume16
Issue number1
Publication statusPublished - 1 Feb 2003

Keywords

  • Association
  • Dopamine D2 receptor
  • Obesity
  • Polymorphism

ASJC Scopus subject areas

  • Internal Medicine
  • Medicine (miscellaneous)
  • Food Science
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Southon, A., Walder, K., Sanigorski, A. M., Zimmet, P., Nicholson, G. C., Kotowicz, M. A., ... Edwards, B. (2003). The taq IA and Ser311Cys polymorphisms in the dopamine D2 receptor gene and obesity. Diabetes, Nutrition and Metabolism - Clinical and Experimental, 16(1), 72-76.