The role of Npas4 in brain development

Martin Lewis, Colleen Ann Bindloss, Thomas Klarić, Brian Key, Simon Koblar

Research output: Contribution to conferencePaper

Abstract

Purpose: To elucidate the developmental role a NPAS4 which is a member of the neural transcription factor bHLH / PAS family. These factors have key roles during development and in the mature organism. NPAS4 expression is restricted to brain, with minor levels detected in testes. NPAS4 expression is specifically expressed in the neurogenic regions of the brain. In adults NPAS4 expression is induced in response to environmental stresses such as ischaemia and seizure. The interest of this study is the role of NPAS4 in the developing embryo. Methods: Zebrafish embryos (n=100s) were injected with three different NPAS4 antisense morpholino oligonucleotides at the single cell stage to reduce expression. Markers of brain development were analysed by whole-mount in-situ hybridisation. Results: Firstly, NPAS4 expression was detected in the developing brain of Danio rerio. Zebrafish embryos at 24hpf displayed reduced brain size and organisation. Morphogens responsible for patterning and neural cell fates had altered expression levels as a result of NPAS4 knock-down. The change in expression patterns of shh and dlx molecules supported the observed morphant phenotype of a reduction in development of the diencephalon. Conclusion: NPAS4 does have a developmental role in forebrain.

Conference

ConferenceAustralasian Neuroscience Society Annual Meeting
Abbreviated titleANS
CountryAustralia
CityHobart
Period27/01/0830/01/08
Internet address

Cite this

Lewis, M., Bindloss, C. A., Klarić, T., Key, B., & Koblar, S. (2008). The role of Npas4 in brain development. Paper presented at Australasian Neuroscience Society Annual Meeting, Hobart, Australia.
Lewis, Martin ; Bindloss, Colleen Ann ; Klarić, Thomas ; Key, Brian ; Koblar, Simon. / The role of Npas4 in brain development. Paper presented at Australasian Neuroscience Society Annual Meeting, Hobart, Australia.
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author = "Martin Lewis and Bindloss, {Colleen Ann} and Thomas Klarić and Brian Key and Simon Koblar",
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Lewis, M, Bindloss, CA, Klarić, T, Key, B & Koblar, S 2008, 'The role of Npas4 in brain development' Paper presented at Australasian Neuroscience Society Annual Meeting, Hobart, Australia, 27/01/08 - 30/01/08, .

The role of Npas4 in brain development. / Lewis, Martin; Bindloss, Colleen Ann; Klarić, Thomas; Key, Brian; Koblar, Simon.

2008. Paper presented at Australasian Neuroscience Society Annual Meeting, Hobart, Australia.

Research output: Contribution to conferencePaper

TY - CONF

T1 - The role of Npas4 in brain development

AU - Lewis, Martin

AU - Bindloss, Colleen Ann

AU - Klarić, Thomas

AU - Key, Brian

AU - Koblar, Simon

PY - 2008

Y1 - 2008

N2 - Purpose: To elucidate the developmental role a NPAS4 which is a member of the neural transcription factor bHLH / PAS family. These factors have key roles during development and in the mature organism. NPAS4 expression is restricted to brain, with minor levels detected in testes. NPAS4 expression is specifically expressed in the neurogenic regions of the brain. In adults NPAS4 expression is induced in response to environmental stresses such as ischaemia and seizure. The interest of this study is the role of NPAS4 in the developing embryo. Methods: Zebrafish embryos (n=100s) were injected with three different NPAS4 antisense morpholino oligonucleotides at the single cell stage to reduce expression. Markers of brain development were analysed by whole-mount in-situ hybridisation. Results: Firstly, NPAS4 expression was detected in the developing brain of Danio rerio. Zebrafish embryos at 24hpf displayed reduced brain size and organisation. Morphogens responsible for patterning and neural cell fates had altered expression levels as a result of NPAS4 knock-down. The change in expression patterns of shh and dlx molecules supported the observed morphant phenotype of a reduction in development of the diencephalon. Conclusion: NPAS4 does have a developmental role in forebrain.

AB - Purpose: To elucidate the developmental role a NPAS4 which is a member of the neural transcription factor bHLH / PAS family. These factors have key roles during development and in the mature organism. NPAS4 expression is restricted to brain, with minor levels detected in testes. NPAS4 expression is specifically expressed in the neurogenic regions of the brain. In adults NPAS4 expression is induced in response to environmental stresses such as ischaemia and seizure. The interest of this study is the role of NPAS4 in the developing embryo. Methods: Zebrafish embryos (n=100s) were injected with three different NPAS4 antisense morpholino oligonucleotides at the single cell stage to reduce expression. Markers of brain development were analysed by whole-mount in-situ hybridisation. Results: Firstly, NPAS4 expression was detected in the developing brain of Danio rerio. Zebrafish embryos at 24hpf displayed reduced brain size and organisation. Morphogens responsible for patterning and neural cell fates had altered expression levels as a result of NPAS4 knock-down. The change in expression patterns of shh and dlx molecules supported the observed morphant phenotype of a reduction in development of the diencephalon. Conclusion: NPAS4 does have a developmental role in forebrain.

M3 - Paper

ER -

Lewis M, Bindloss CA, Klarić T, Key B, Koblar S. The role of Npas4 in brain development. 2008. Paper presented at Australasian Neuroscience Society Annual Meeting, Hobart, Australia.