The role of cell surface markers and enamel matrix derivatives on human periodontal ligament mesenchymal progenitor responses in vitro

Philippe Kémoun, Stan Gronthos, Malcolm L. Snead, Jacqueline Rue, Bruno Courtois, Frédéric Vaysse, Jean Pierre Salles, Gérard Brunel

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Periodontitis is a chronic-, infectious-disease of the human periodontium that is characterized by the loss of supporting tissues surrounding the tooth such as the periodontal ligament (PDL), cementum and alveolar bone. Regeneration of the periodontium is dependent on the participation of mesenchymal stem/stromal cells (MSC) resident in the PDL. Enamel matrix derivative (EMD), an extract from immature porcine enamel rich in amelogenin protein but that also contain bone morphogenetic protein (BMP), is used to treat periodontal defects. The effects of EMD on MSC cells of the PDL are not well characterized. In this in vitro study, we identify PDL progenitor cells from multiple individuals and demonstrate that EMD stimulates them. We show that the effect of EMD on cell proliferation and migration is mediated through the amelogenin it contains, while the differentiation of these progenitor cells to cell types of mineralized tissue is mainly due to BMP signaling.

LanguageEnglish
Pages7375-7388
Number of pages14
JournalBiomaterials
Volume32
Issue number30
DOIs
Publication statusPublished - 1 Oct 2011

Keywords

  • Amelogenin
  • Bone morphogenetic protein
  • Enamel matrix derivative (EMD)
  • Mesenchymal stem cell
  • Periodontium

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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