The pentatricopeptide repeat protein EMB2654 is essential for trans-splicing of a chloroplast small ribosomal subunit transcript

Nader Aryamanesh, Hannes Ruwe, Lilian Vincis Pereira Sanglard, Leila Eshraghi, John D. Bussell, Katharine A. Howell, Ian Small, Catherine Colas des Francs-Small

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We report the partial complementation and subsequent comparative molecular analysis of two nonviable mutants impaired in chloroplast translation, one (emb2394) lacking the RPL6 protein, and the other (emb2654) carrying a mutation in a gene encoding a P-class pentatricopeptide repeat protein. We show that EMB2654 is required for the trans-splicing of the plastid rps12 transcript and that therefore the emb2654 mutant lacks Rps12 protein and fails to assemble the small subunit of the plastid ribosome, explaining the loss of plastid translation and consequent embryo-lethal phenotype. Predictions of the EMB2654 binding site match a small RNA “footprint” located on the 59 half of the trans-spliced intron that is almost absent in the partially complemented mutant. EMB2654 binds sequence specifically to this target sequence in vitro. Altered patterns in nucleaseprotected small RNA fragments in emb2654 show that EMB2654 binding must be an early step in, or prior to, the formation of a large protein-RNA complex covering the free ends of the two rps12 intron halves.

LanguageEnglish
Pages1164-1176
Number of pages13
JournalPlant Physiology
Volume173
Issue number2
DOIs
Publication statusPublished - 1 Feb 2017
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Aryamanesh, N., Ruwe, H., Sanglard, L. V. P., Eshraghi, L., Bussell, J. D., Howell, K. A., ... des Francs-Small, C. C. (2017). The pentatricopeptide repeat protein EMB2654 is essential for trans-splicing of a chloroplast small ribosomal subunit transcript. Plant Physiology, 173(2), 1164-1176. https://doi.org/10.1104/pp.16.01840
Aryamanesh, Nader ; Ruwe, Hannes ; Sanglard, Lilian Vincis Pereira ; Eshraghi, Leila ; Bussell, John D. ; Howell, Katharine A. ; Small, Ian ; des Francs-Small, Catherine Colas. / The pentatricopeptide repeat protein EMB2654 is essential for trans-splicing of a chloroplast small ribosomal subunit transcript. In: Plant Physiology. 2017 ; Vol. 173, No. 2. pp. 1164-1176.
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Aryamanesh, N, Ruwe, H, Sanglard, LVP, Eshraghi, L, Bussell, JD, Howell, KA, Small, I & des Francs-Small, CC 2017, 'The pentatricopeptide repeat protein EMB2654 is essential for trans-splicing of a chloroplast small ribosomal subunit transcript', Plant Physiology, vol. 173, no. 2, pp. 1164-1176. https://doi.org/10.1104/pp.16.01840

The pentatricopeptide repeat protein EMB2654 is essential for trans-splicing of a chloroplast small ribosomal subunit transcript. / Aryamanesh, Nader; Ruwe, Hannes; Sanglard, Lilian Vincis Pereira; Eshraghi, Leila; Bussell, John D.; Howell, Katharine A.; Small, Ian; des Francs-Small, Catherine Colas.

In: Plant Physiology, Vol. 173, No. 2, 01.02.2017, p. 1164-1176.

Research output: Contribution to journalArticle

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