The olsA gene mediates the synthesis of an ornithine lipid in Pseudomonas aeruginosa during growth under phosphate-limiting conditions, but is not involved in antimicrobial peptide susceptibility

Shawn Lewenza, Reza Falsafi, Manjeet Bains, Patricia Rohs, Jacek Stupak, G. Dennis Sprott, Robert E.W. Hancock

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20 Citations (Scopus)


Pseudomonas aeruginosa responds to phosphate limitation by inducing the expression of phosphate transport systems, phosphatases, hemolysins and a DNase, many of which are important for virulence. Here we report that under phosphate-limiting conditions, P. aeruginosa produces a phosphate-free ornithine lipid (OL) as the primary membrane lipid. The olsBA (PA4350-PA4351) genes were highly induced under phosphate-limiting conditions. The production and structure of the OL was confirmed by MS, revealing diagnostic fragment ions and mainly C16:0 and C18:1 dialkyl chains. It was shown that olsA is required for production of these lipids and genetic complementation of the olsA{proportion}lux mutant restored OL production. Studies in other bacteria have correlated increased resistance to antimicrobial peptides with the production of OLs. Here it was demonstrated that resistance to antimicrobial peptides increased under phosphate-limiting conditions, but OLs were not required for this increased resistance. OL production was also not required for virulence in the Caenorhabditis elegans infection model. The production of OLs is a strategy to reduce phosphate utilization in the membrane, but mutants unable to produce OLs have no observable phenotype with respect to growth, antibiotic resistance or virulence.

Original languageEnglish
Pages (from-to)95-102
Number of pages8
JournalFEMS Microbiology Letters
Issue number2
Publication statusPublished - Jul 2011


  • Antibiotic resistance
  • Bacterial membranes
  • Environmental sensing

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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