The in vivo and in vitro effects of exogenous leptin on ovulation in the rat

Priya S. Duggal, Kylie H. Van Der Hoek, Clyde R. Milner, Natalie Ryan, David T. Armstrong, Denis A. Magoffin, Robert J. Norman

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148 Citations (Scopus)

Abstract

Leptin, a hormonal product of the Lep gene, is expressed by adipocytes and is thought to play a role in regulating food intake and reproduction. The leptin protein has been localized in many reproductive tissues, including the ovary. Several publications indicate that the ovary is directly affected by leptin and that leptin may be a factor linking obesity and reproductive dysfunction. In this study, the effect of systemic leptin administration on ovulation in the rat ovary, both in vivo and in vitro, was investigated. Ip administration of leptin (30 μg at 3 hourly intervals for 15 h) to immature gonadotropinprimed rats caused a decline in ovulation in vivo, from 15.9 ± 2.0 oocytes in the control animals to 5.3 ± 1.6 oocytes in the leptin-treated animals (P < 0.001). Plasma progesterone and estradiol levels were analyzed immediately before ovulation, and neither was altered significantly in animals receiving the leptin treatment. Food consumption and body weight decreased following leptin treatment; however, a loss in body weight alone (pair-fed controls) was insufficient to explain the decrease in ovulation observed in the leptin-treated animals. In vitro perfusion of FSH-primed whole ovaries showed that treatment with leptin in combination with LH significantly decreased ovulations from 5.7 ± 1.6 per ovary perfused with LH alone to 1.3 ± 0.6 in those with LH and 1 μg/ml leptin (P < 0.05). Progesterone and estradiol levels in the samples taken during the perfusion period were unaffected by leptin treatment. In summary, leptin administration resulted in fewer ovulations, both in vivo and in vitro, but did not influence steroid levels. Systemic leptin administration at these doses can therefore inhibit ovulation, a process that occurs through a direct effect on the ovary.

LanguageEnglish
Pages1971-1976
Number of pages6
JournalEndocrinology
Volume141
Issue number6
DOIs
Publication statusPublished - 1 Jan 2000
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology

Cite this

Duggal, P. S., Van Der Hoek, K. H., Milner, C. R., Ryan, N., Armstrong, D. T., Magoffin, D. A., & Norman, R. J. (2000). The in vivo and in vitro effects of exogenous leptin on ovulation in the rat. Endocrinology, 141(6), 1971-1976. https://doi.org/10.1210/endo.141.6.7509
Duggal, Priya S. ; Van Der Hoek, Kylie H. ; Milner, Clyde R. ; Ryan, Natalie ; Armstrong, David T. ; Magoffin, Denis A. ; Norman, Robert J. / The in vivo and in vitro effects of exogenous leptin on ovulation in the rat. In: Endocrinology. 2000 ; Vol. 141, No. 6. pp. 1971-1976.
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Duggal, PS, Van Der Hoek, KH, Milner, CR, Ryan, N, Armstrong, DT, Magoffin, DA & Norman, RJ 2000, 'The in vivo and in vitro effects of exogenous leptin on ovulation in the rat', Endocrinology, vol. 141, no. 6, pp. 1971-1976. https://doi.org/10.1210/endo.141.6.7509

The in vivo and in vitro effects of exogenous leptin on ovulation in the rat. / Duggal, Priya S.; Van Der Hoek, Kylie H.; Milner, Clyde R.; Ryan, Natalie; Armstrong, David T.; Magoffin, Denis A.; Norman, Robert J.

In: Endocrinology, Vol. 141, No. 6, 01.01.2000, p. 1971-1976.

Research output: Contribution to journalArticle

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AU - Duggal, Priya S.

AU - Van Der Hoek, Kylie H.

AU - Milner, Clyde R.

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Duggal PS, Van Der Hoek KH, Milner CR, Ryan N, Armstrong DT, Magoffin DA et al. The in vivo and in vitro effects of exogenous leptin on ovulation in the rat. Endocrinology. 2000 Jan 1;141(6):1971-1976. https://doi.org/10.1210/endo.141.6.7509