The effect of suramin on vasodilator responses to ATP and 2-methylthio-ATP in the Sprague-Dawley rat coronary vasculature

Amanda J. Vials, Geoffrey Burnstock

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The effect of suramin, a P2 purinoceptor antagonist, on the vasodilator response to adenosine 5′-triphosphate (ATP), 2-methylthio-ATP (2-meSATP) and adenosine were examined in the Sprague-Dawley rat coronary vasculature using the Langendorff heart preparation. Relaxation induced by 2-meSATP was significantly inhibited by suramin. Only responses to low doses of adenosine and ATP were inhibited bu suramin. 8-(p-Sulphophenyl)theophylline (8-PSPT) did not affect the relaxant response to ATP and 2-meSATP at a concentration that significantly inhibited the response to adenosine. It is concluded that 2-meSATP acts via P2Y purinoceptors while ATP appears to be acting largely through a different mechanism. It is not acting via a P1 purinoceptor because ATP was not inhibited by the P1 purinoceptor antagonist 8-PSPT.

LanguageEnglish
Pages299-302
Number of pages4
JournalEuropean Journal of Pharmacology
Volume251
Issue number2-3
DOIs
Publication statusPublished - 14 Jan 1994

Keywords

  • Coronary vasculature
  • P purinoceptor
  • Suramin

ASJC Scopus subject areas

  • Pharmacology

Cite this

@article{35869a5bfd0e4c738f66c2542eec25bc,
title = "The effect of suramin on vasodilator responses to ATP and 2-methylthio-ATP in the Sprague-Dawley rat coronary vasculature",
abstract = "The effect of suramin, a P2 purinoceptor antagonist, on the vasodilator response to adenosine 5′-triphosphate (ATP), 2-methylthio-ATP (2-meSATP) and adenosine were examined in the Sprague-Dawley rat coronary vasculature using the Langendorff heart preparation. Relaxation induced by 2-meSATP was significantly inhibited by suramin. Only responses to low doses of adenosine and ATP were inhibited bu suramin. 8-(p-Sulphophenyl)theophylline (8-PSPT) did not affect the relaxant response to ATP and 2-meSATP at a concentration that significantly inhibited the response to adenosine. It is concluded that 2-meSATP acts via P2Y purinoceptors while ATP appears to be acting largely through a different mechanism. It is not acting via a P1 purinoceptor because ATP was not inhibited by the P1 purinoceptor antagonist 8-PSPT.",
keywords = "Coronary vasculature, P purinoceptor, Suramin",
author = "Vials, {Amanda J.} and Geoffrey Burnstock",
year = "1994",
month = "1",
day = "14",
doi = "10.1016/0014-2999(94)90413-8",
language = "English",
volume = "251",
pages = "299--302",
journal = "European Journal of Pharmacology",
issn = "0014-2999",
publisher = "Elsevier",
number = "2-3",

}

The effect of suramin on vasodilator responses to ATP and 2-methylthio-ATP in the Sprague-Dawley rat coronary vasculature. / Vials, Amanda J.; Burnstock, Geoffrey.

In: European Journal of Pharmacology, Vol. 251, No. 2-3, 14.01.1994, p. 299-302.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The effect of suramin on vasodilator responses to ATP and 2-methylthio-ATP in the Sprague-Dawley rat coronary vasculature

AU - Vials, Amanda J.

AU - Burnstock, Geoffrey

PY - 1994/1/14

Y1 - 1994/1/14

N2 - The effect of suramin, a P2 purinoceptor antagonist, on the vasodilator response to adenosine 5′-triphosphate (ATP), 2-methylthio-ATP (2-meSATP) and adenosine were examined in the Sprague-Dawley rat coronary vasculature using the Langendorff heart preparation. Relaxation induced by 2-meSATP was significantly inhibited by suramin. Only responses to low doses of adenosine and ATP were inhibited bu suramin. 8-(p-Sulphophenyl)theophylline (8-PSPT) did not affect the relaxant response to ATP and 2-meSATP at a concentration that significantly inhibited the response to adenosine. It is concluded that 2-meSATP acts via P2Y purinoceptors while ATP appears to be acting largely through a different mechanism. It is not acting via a P1 purinoceptor because ATP was not inhibited by the P1 purinoceptor antagonist 8-PSPT.

AB - The effect of suramin, a P2 purinoceptor antagonist, on the vasodilator response to adenosine 5′-triphosphate (ATP), 2-methylthio-ATP (2-meSATP) and adenosine were examined in the Sprague-Dawley rat coronary vasculature using the Langendorff heart preparation. Relaxation induced by 2-meSATP was significantly inhibited by suramin. Only responses to low doses of adenosine and ATP were inhibited bu suramin. 8-(p-Sulphophenyl)theophylline (8-PSPT) did not affect the relaxant response to ATP and 2-meSATP at a concentration that significantly inhibited the response to adenosine. It is concluded that 2-meSATP acts via P2Y purinoceptors while ATP appears to be acting largely through a different mechanism. It is not acting via a P1 purinoceptor because ATP was not inhibited by the P1 purinoceptor antagonist 8-PSPT.

KW - Coronary vasculature

KW - P purinoceptor

KW - Suramin

UR - http://www.scopus.com/inward/record.url?scp=0028006119&partnerID=8YFLogxK

U2 - 10.1016/0014-2999(94)90413-8

DO - 10.1016/0014-2999(94)90413-8

M3 - Article

VL - 251

SP - 299

EP - 302

JO - European Journal of Pharmacology

T2 - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

IS - 2-3

ER -