The effect of short-term overfeeding on serum lipids in healthy humans

Leonie Heilbronn, Adelle C.F. Coster, Lesley V. Campbell, Jerry R. Greenfield, Kylie Lange, Michael J. Christopher, Peter J. Meikle, Dorit Samocha-Bonet

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Objectives While chronic obesity is associated with alterations in circulating glycerolipids, sphingolipids and plasmalogens, the effects of short-term overfeeding in humans are unclear. Design and Methods Healthy individuals (n = 40) were overfed by 1,250 kcal day-1 for 28 days. Insulin sensitivity (hyperinsulinemic-euglycemic clamp), abdominal fat distribution and serum lipidomics (mass spectrometry) were assessed. Results Overfeeding increased liver fat, insulin resistance, serum C-reactive protein and urinary F2-isoprostanes. HDL increased (11% ± 2%, P < 0.001) while LDL, triglycerides and nonesterified fatty acids were unchanged. Three hundred and thirty three serum lipids were detected, of which 13% increased and 20% decreased with overfeeding. Total diacylglycerol and lysoalkylphosphatidylcholine (LPC(O)) concentrations decreased (P < 0.01), while total ceramide, Cer22:0 and Cer24:0 increased (P ≤ 0.01). The most notable increases were observed in the HDL-associated phosphatidylethanolamine- based plasmalogens and their precursors alkylhosphatidylethanolamine (18 ± 5% and 38 ± 8% respectively, P ≤ 0.01). Conclusions Overfeeding led to weight gain and changes in the serum lipid profile. Increases in ceramides were noted, which left unchecked may promote systemic insulin resistance. Uniform increases were observed in plasmalogens and their precursors. Because plasmalogens are powerful antioxidants, this may be an appropriate response against increased oxidative stress generated by over-nutrition. The metabolic consequences of changes in concentrations of many circulating lipid species with overfeeding require further study.

LanguageEnglish
JournalObesity
Volume21
Issue number12
DOIs
Publication statusPublished - 1 Dec 2013

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Nutrition and Dietetics

Cite this

Heilbronn, L., Coster, A. C. F., Campbell, L. V., Greenfield, J. R., Lange, K., Christopher, M. J., ... Samocha-Bonet, D. (2013). The effect of short-term overfeeding on serum lipids in healthy humans. Obesity, 21(12). https://doi.org/10.1002/oby.20508
Heilbronn, Leonie ; Coster, Adelle C.F. ; Campbell, Lesley V. ; Greenfield, Jerry R. ; Lange, Kylie ; Christopher, Michael J. ; Meikle, Peter J. ; Samocha-Bonet, Dorit. / The effect of short-term overfeeding on serum lipids in healthy humans. In: Obesity. 2013 ; Vol. 21, No. 12.
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abstract = "Objectives While chronic obesity is associated with alterations in circulating glycerolipids, sphingolipids and plasmalogens, the effects of short-term overfeeding in humans are unclear. Design and Methods Healthy individuals (n = 40) were overfed by 1,250 kcal day-1 for 28 days. Insulin sensitivity (hyperinsulinemic-euglycemic clamp), abdominal fat distribution and serum lipidomics (mass spectrometry) were assessed. Results Overfeeding increased liver fat, insulin resistance, serum C-reactive protein and urinary F2-isoprostanes. HDL increased (11{\%} ± 2{\%}, P < 0.001) while LDL, triglycerides and nonesterified fatty acids were unchanged. Three hundred and thirty three serum lipids were detected, of which 13{\%} increased and 20{\%} decreased with overfeeding. Total diacylglycerol and lysoalkylphosphatidylcholine (LPC(O)) concentrations decreased (P < 0.01), while total ceramide, Cer22:0 and Cer24:0 increased (P ≤ 0.01). The most notable increases were observed in the HDL-associated phosphatidylethanolamine- based plasmalogens and their precursors alkylhosphatidylethanolamine (18 ± 5{\%} and 38 ± 8{\%} respectively, P ≤ 0.01). Conclusions Overfeeding led to weight gain and changes in the serum lipid profile. Increases in ceramides were noted, which left unchecked may promote systemic insulin resistance. Uniform increases were observed in plasmalogens and their precursors. Because plasmalogens are powerful antioxidants, this may be an appropriate response against increased oxidative stress generated by over-nutrition. The metabolic consequences of changes in concentrations of many circulating lipid species with overfeeding require further study.",
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Heilbronn, L, Coster, ACF, Campbell, LV, Greenfield, JR, Lange, K, Christopher, MJ, Meikle, PJ & Samocha-Bonet, D 2013, 'The effect of short-term overfeeding on serum lipids in healthy humans', Obesity, vol. 21, no. 12. https://doi.org/10.1002/oby.20508

The effect of short-term overfeeding on serum lipids in healthy humans. / Heilbronn, Leonie; Coster, Adelle C.F.; Campbell, Lesley V.; Greenfield, Jerry R.; Lange, Kylie; Christopher, Michael J.; Meikle, Peter J.; Samocha-Bonet, Dorit.

In: Obesity, Vol. 21, No. 12, 01.12.2013.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The effect of short-term overfeeding on serum lipids in healthy humans

AU - Heilbronn, Leonie

AU - Coster, Adelle C.F.

AU - Campbell, Lesley V.

AU - Greenfield, Jerry R.

AU - Lange, Kylie

AU - Christopher, Michael J.

AU - Meikle, Peter J.

AU - Samocha-Bonet, Dorit

PY - 2013/12/1

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N2 - Objectives While chronic obesity is associated with alterations in circulating glycerolipids, sphingolipids and plasmalogens, the effects of short-term overfeeding in humans are unclear. Design and Methods Healthy individuals (n = 40) were overfed by 1,250 kcal day-1 for 28 days. Insulin sensitivity (hyperinsulinemic-euglycemic clamp), abdominal fat distribution and serum lipidomics (mass spectrometry) were assessed. Results Overfeeding increased liver fat, insulin resistance, serum C-reactive protein and urinary F2-isoprostanes. HDL increased (11% ± 2%, P < 0.001) while LDL, triglycerides and nonesterified fatty acids were unchanged. Three hundred and thirty three serum lipids were detected, of which 13% increased and 20% decreased with overfeeding. Total diacylglycerol and lysoalkylphosphatidylcholine (LPC(O)) concentrations decreased (P < 0.01), while total ceramide, Cer22:0 and Cer24:0 increased (P ≤ 0.01). The most notable increases were observed in the HDL-associated phosphatidylethanolamine- based plasmalogens and their precursors alkylhosphatidylethanolamine (18 ± 5% and 38 ± 8% respectively, P ≤ 0.01). Conclusions Overfeeding led to weight gain and changes in the serum lipid profile. Increases in ceramides were noted, which left unchecked may promote systemic insulin resistance. Uniform increases were observed in plasmalogens and their precursors. Because plasmalogens are powerful antioxidants, this may be an appropriate response against increased oxidative stress generated by over-nutrition. The metabolic consequences of changes in concentrations of many circulating lipid species with overfeeding require further study.

AB - Objectives While chronic obesity is associated with alterations in circulating glycerolipids, sphingolipids and plasmalogens, the effects of short-term overfeeding in humans are unclear. Design and Methods Healthy individuals (n = 40) were overfed by 1,250 kcal day-1 for 28 days. Insulin sensitivity (hyperinsulinemic-euglycemic clamp), abdominal fat distribution and serum lipidomics (mass spectrometry) were assessed. Results Overfeeding increased liver fat, insulin resistance, serum C-reactive protein and urinary F2-isoprostanes. HDL increased (11% ± 2%, P < 0.001) while LDL, triglycerides and nonesterified fatty acids were unchanged. Three hundred and thirty three serum lipids were detected, of which 13% increased and 20% decreased with overfeeding. Total diacylglycerol and lysoalkylphosphatidylcholine (LPC(O)) concentrations decreased (P < 0.01), while total ceramide, Cer22:0 and Cer24:0 increased (P ≤ 0.01). The most notable increases were observed in the HDL-associated phosphatidylethanolamine- based plasmalogens and their precursors alkylhosphatidylethanolamine (18 ± 5% and 38 ± 8% respectively, P ≤ 0.01). Conclusions Overfeeding led to weight gain and changes in the serum lipid profile. Increases in ceramides were noted, which left unchecked may promote systemic insulin resistance. Uniform increases were observed in plasmalogens and their precursors. Because plasmalogens are powerful antioxidants, this may be an appropriate response against increased oxidative stress generated by over-nutrition. The metabolic consequences of changes in concentrations of many circulating lipid species with overfeeding require further study.

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Heilbronn L, Coster ACF, Campbell LV, Greenfield JR, Lange K, Christopher MJ et al. The effect of short-term overfeeding on serum lipids in healthy humans. Obesity. 2013 Dec 1;21(12). https://doi.org/10.1002/oby.20508