The antimicrobial peptide polyphemusin localizes to the cytoplasm of Escherichia coli following treatment

Jon Paul S. Powers, Morgan M. Martin, Danika L. Goosney, Robert E.W. Hancock

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)


The horseshoe crab peptide polyphemusin I possesses high antimicrobial activity, but its mechanism of action is as yet not well defined. Using a biotin-labeled polyphemusin I analogue and confocal fluorescence microscopy, we showed that the peptide accumulates in the cytoplasm of wild-type Escherichia coli within 30 min after addition without causing substantial membrane damage.

Original languageEnglish
Pages (from-to)1522-1524
Number of pages3
JournalAntimicrobial Agents and Chemotherapy
Issue number4
Publication statusPublished - 1 Apr 2006
Externally publishedYes

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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