Systematic Evolution Of Ligands By Exponential Enrichment: Photoselection Of Nucleic Acid Ligands And Solution Selex

Kirk Jensen (Inventor)

Research output: Patent

Abstract

A method for identifying nucleic acid ligands to target molecules using the SELEX procedure wherein the candidate nucleic acids contain photoreactive groups and nucleic acid ligands identified thereby are claimed. The complexes of increased affinity nucleic acids and target molecules formed in the procedure are crosslinked by irradiation to facilitate separation from unbound nucleic acids. In other methods partitioning of high and low affinity nucleic acids is facilitated by primer extension steps as shown in the figure in which chain termination nucleotides, digestion resistant nucleotides or nucleotides that allow retention of the cDNA product on an affinity matrix are differentially incorporated into the cDNA products of either the high or low affinity nucleic acids and the cDNA products are treated accordingly to amplification, enzymatic or chemical digestion or by contact with an affinity matrix.
LanguageEnglish
Patent numberUS 6291184 B1
IPCUS 6291184 B1
Publication statusPublished - 18 Sep 2001

Cite this

Jensen, K. (2001). IPC No. US 6291184 B1. Systematic Evolution Of Ligands By Exponential Enrichment: Photoselection Of Nucleic Acid Ligands And Solution Selex. (Patent No. US 6291184 B1). United States Patent & Trademark Office.
Jensen, Kirk (Inventor). / Systematic Evolution Of Ligands By Exponential Enrichment: Photoselection Of Nucleic Acid Ligands And Solution Selex. United States Patent & Trademark Office. IPC No.: US 6291184 B1. Patent No.: US 6291184 B1.
@misc{38e87f7990dc466ea7b5d6a1bc97e932,
title = "Systematic Evolution Of Ligands By Exponential Enrichment: Photoselection Of Nucleic Acid Ligands And Solution Selex",
abstract = "A method for identifying nucleic acid ligands to target molecules using the SELEX procedure wherein the candidate nucleic acids contain photoreactive groups and nucleic acid ligands identified thereby are claimed. The complexes of increased affinity nucleic acids and target molecules formed in the procedure are crosslinked by irradiation to facilitate separation from unbound nucleic acids. In other methods partitioning of high and low affinity nucleic acids is facilitated by primer extension steps as shown in the figure in which chain termination nucleotides, digestion resistant nucleotides or nucleotides that allow retention of the cDNA product on an affinity matrix are differentially incorporated into the cDNA products of either the high or low affinity nucleic acids and the cDNA products are treated accordingly to amplification, enzymatic or chemical digestion or by contact with an affinity matrix.",
author = "Kirk Jensen",
year = "2001",
month = "9",
day = "18",
language = "English",
publisher = "United States Patent & Trademark Office",
type = "Patent",
note = "US 6291184 B1; US 6291184 B1",

}

Systematic Evolution Of Ligands By Exponential Enrichment: Photoselection Of Nucleic Acid Ligands And Solution Selex. / Jensen, Kirk (Inventor).

United States Patent & Trademark Office. IPC No.: US 6291184 B1. Patent No.: US 6291184 B1.

Research output: Patent

TY - PAT

T1 - Systematic Evolution Of Ligands By Exponential Enrichment: Photoselection Of Nucleic Acid Ligands And Solution Selex

AU - Jensen, Kirk

PY - 2001/9/18

Y1 - 2001/9/18

N2 - A method for identifying nucleic acid ligands to target molecules using the SELEX procedure wherein the candidate nucleic acids contain photoreactive groups and nucleic acid ligands identified thereby are claimed. The complexes of increased affinity nucleic acids and target molecules formed in the procedure are crosslinked by irradiation to facilitate separation from unbound nucleic acids. In other methods partitioning of high and low affinity nucleic acids is facilitated by primer extension steps as shown in the figure in which chain termination nucleotides, digestion resistant nucleotides or nucleotides that allow retention of the cDNA product on an affinity matrix are differentially incorporated into the cDNA products of either the high or low affinity nucleic acids and the cDNA products are treated accordingly to amplification, enzymatic or chemical digestion or by contact with an affinity matrix.

AB - A method for identifying nucleic acid ligands to target molecules using the SELEX procedure wherein the candidate nucleic acids contain photoreactive groups and nucleic acid ligands identified thereby are claimed. The complexes of increased affinity nucleic acids and target molecules formed in the procedure are crosslinked by irradiation to facilitate separation from unbound nucleic acids. In other methods partitioning of high and low affinity nucleic acids is facilitated by primer extension steps as shown in the figure in which chain termination nucleotides, digestion resistant nucleotides or nucleotides that allow retention of the cDNA product on an affinity matrix are differentially incorporated into the cDNA products of either the high or low affinity nucleic acids and the cDNA products are treated accordingly to amplification, enzymatic or chemical digestion or by contact with an affinity matrix.

M3 - Patent

M1 - US 6291184 B1

PB - United States Patent & Trademark Office

ER -