Synthesis of peptide arrays using SPOT-technology and the CelluSpots-method

Dirk F.H. Winkler, Kai Hilpert, Ole Brandt, Robert Hancock

Research output: Chapter in Book/Report/Conference proceedingChapter

45 Citations (Scopus)

Abstract

Peptide synthesis on cellulose using the SPOT technology follows the standard Fmoc-chemistry and can be performed manually or automated. This method allows the synthesis of low-cost peptide arrays containing around 900 large spots of addressable peptides on a cellulose sheet of 19 cm × 29 cm. These peptides can be cleaved from the cellulose support by ammonia gas and afterward spotted on glass microchips. Alternatively, the peptides can be synthesized on modified cellulose discs and CelluSpot microarrays can be produced.

Original languageEnglish
Title of host publicationPeptide Microarrays
Subtitle of host publicationMethods and Protocols
EditorsMarina Cretich, Marcella Chiari
Pages157-174
Number of pages18
DOIs
Publication statusPublished - 1 Dec 2009
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume570
ISSN (Print)1064-3745

Keywords

  • Spot synthesis
  • peptide array
  • screening

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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