Survival and engraftment of mouse embryonic stem cell-derived implants in the guinea pig brain

A. J. Robinson, A. C. Meedeniya, K. M. Hemsley, D. Auclair, A. C. Crawley, J. J. Hopwood

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

α-Mannosidosis is a lysosomal storage disease resulting from a deficiency of the enzyme α-d-mannosidase. A major feature of α-mannosidosis is progressive neurological decline, for which there is no safe and effective treatment available. We have a guinea pig model of α-mannosidosis that models the human condition. This study investigates the feasibility of implanting differentiated mouse embryonic stem cells in the neonatal guinea pig brain in order to provide a source of α-mannosidase to the affected central nervous system. Cells implanted at a low dose (1.5 × 103 cells per hemisphere) at 1 week of age were found to survive in very low numbers in some immunosuppressed animals out to 8 weeks. Four weeks post-implantation, cells implanted in high numbers (105 cells per hemisphere) formed teratomas in the majority of the animals implanted. Although implanted cells were found to migrate extensively within the brain and differentiate into mature cells of neural (and other) lineages, the safety issue related to uncontrolled cell proliferation precluded the use of this cell type for longer-term implantation studies. We conclude that the pluripotent cell type used in this study is unsuitable for achieving safe engraftment in the guinea pig brain.

Original languageEnglish
Pages (from-to)161-168
Number of pages8
JournalNeuroscience Research
Volume53
Issue number2
DOIs
Publication statusPublished - 1 Oct 2005

Keywords

  • Differentiation
  • Guinea pig
  • Lysosomal storage disease
  • Mouse embryonic stem cells
  • Stem cell implantation
  • Stem cell therapy
  • α-Mannosidosis

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Robinson, A. J., Meedeniya, A. C., Hemsley, K. M., Auclair, D., Crawley, A. C., & Hopwood, J. J. (2005). Survival and engraftment of mouse embryonic stem cell-derived implants in the guinea pig brain. Neuroscience Research, 53(2), 161-168. https://doi.org/10.1016/j.neures.2005.06.010