Surface modification of bioceramics affect osteoblastic cells response

H. Zreiqat, C. R. Howlett, A. Zannettino, P. Evans, C. Knabe, G. Schulze-Tanzil, G. M. Shakibaei

Research output: Contribution to journalConference articlepeer-review

4 Citations (Scopus)


Poor cell adhesion to orthopaedic and dental implants may result in implant failure. Cellular adhesion to biomaterial surfaces is primarily mediated by integrins, which act as signal transduction and adhesion proteins. Since integrin function depends on divalent cations, we investigated the effect of magnesium ions modified bioceramic substrata (Al2O3-Mg2+) on human bone-derived cell (HBDC) adhesion, integrin expression and activation of intracellular signalling molecules. Our findings demonstrated that adhesion, spreading and expression of extracellular matrix proteins was increased when HBDC were cultured on Al2O3-Mg2+ compared to the Mg2+-free Al2O3. Moreover, these cells grown on Al2O3-Mg2+ expressed significantly enhanced levels of β1-, α5β1- and α3β1-integrins receptors. Tyrosine phosphorylation of intracellular integrin-dependent signalling proteins and She isoforms (p46, p52, p66) were significantly enhanced when HBDC were grown on Al2O3-Mg2+ compared to Al2O3. We demonstrated that Mg2+-promoted cell adhesion depends on α5β1- and β1-integrins associated signal transduction pathways involving the key signalling protein. Since Mg2+-modified substrata enhanced osteoblastic proliferation and differentiation, their potency to enhance osteogenesis is hereby suggested. Mg2+ supplementation of bioceramic substrata may be a promising way to improve integration of implants in orthopaedic and dental surgery.

Original languageEnglish
Pages (from-to)707-710
Number of pages4
JournalKey Engineering Materials
Publication statusPublished or Issued - 1 Jan 2003
Externally publishedYes
EventProceedings of the 15th International Symposium on Ceramics in Medicine; The Annual Meeting of the International Society for Ceramics in Medicine - Sydney, NSW, Australia
Duration: 4 Dec 20028 Dec 2002


  • Adhesion
  • Bioceramic
  • Integrins
  • Magnesium ions
  • Matrix proteins
  • Osteoblasts

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

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