Stimulation of synthesis of free chondroitin sulfate chains by β D xylosides in cultured cells

L. Galligani, J. Hopwood, N. B. Schwartz, A. Dorfman

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Abstract

β Xylosides stimulate 2 to 6 fold the synthesis of glycosaminoglycans by three types of nonconnective tissue cells (RG C6, NB41A, and rat hepatoma cells), and normal and simian virus 40 (SV40) transformed normal human skin fibroblasts. The effect, which is specific for the anomeric linkage and the glycone, is observed in the presence and absence of puromycin. β Xylosides may substitute for xylosylated core protein as initiators of synthesis of chondroitin sulfate chains. No stimulation of synthesis of heparan sulfate was observed. With the use of a fluorogenic xyloside, 4 methylumbelliferyl β D xyloside, it was demonstrated that the free chondroitin sulfate chains secreted into the medium bear the xyloside at the reducing end, and have an average molecular weight of 16,500.

Original languageEnglish
Pages (from-to)5400-5406
Number of pages7
JournalJournal of Biological Chemistry
Volume250
Issue number14
Publication statusPublished or Issued - 1975

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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