Stem cell properties of human dental pulp stem cells

S. Gronthos, J. Brahim, W. Li, L. W. Fisher, N. Cherman, A. Boyde, P. DenBesten, P. Gehron Robey, S. Shi

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In this study, we characterized the self-renewal capability, multi-lineage differentiation capacity, and clonogenic efficiency of human dental pulp stem cells (DPSCs). DPSCs were capable of forming ectopic dentin and associated pulp tissue in vivo. Stromal-like cells were reestablished in culture from primary DPSC transplants and retransplanted into immunocompromised mice to generate a dentin-pulp-like tissue, demonstrating their self-renewal capability. DPSCs were also found to be capable of differentiating into adipocytes and neural-like cells. The odontogenic potential of 12 individual single-colony-derived DPSC strains was determined. Two-thirds of the single-colony-derived DPSC strains generated abundant ectopic dentin in vivo, while only a limited amount of dentin was detected in the remaining one-third. These results indicate that single-colony-derived DPSC strains differ from each other with respect to their rate of odontogenesis. Taken together, these results demonstrate that DPSCs possess stem-cell-like qualities, including self-renewal capability and multi-lineage differentiation.

Original languageEnglish
Pages (from-to)531-535
Number of pages5
JournalJournal of Dental Research
Issue number8
Publication statusPublished - 1 Aug 2002

ASJC Scopus subject areas

  • Dentistry(all)

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