Site-specific pre-evaluation of bioremediation technologies for chloroethene degradation

S. S. Patil, E. M. Adetutu, P. J. Sheppard, P. Morrison, I. R. Menz, A. S. Ball

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Groundwater systems are important sources of water for drinking and irrigation purposes. Unfortunately, human activities have led to widespread groundwater contamination by chlorinated compounds such as tetrachloroethene (PCE). Chloroethenes are extremely harmful to humans and the environment due to their carcinogenic properties. Therefore, this study investigated the potential for bioremediating PCE-contaminated groundwater using laboratory-based biostimulation (BS) and biostimulation–bioaugmentation (BS-BA) assays. This was carried out on groundwater samples obtained from a PCE-contaminated site which had been unsuccessfully treated using chemical oxidation. BS resulted in complete dechlorination by week 21 compared to controls which had only 30 % PCE degradation. BS also led to a approximately threefold increase in 16S rRNA gene copies compared to the controls. However, the major bacterial dechlorinating group, Dehalococcoides (Dhc), was undetectable in PCE-contaminated groundwater. This suggested that dechlorination in BS samples was due to indigenous non-Dhc dechlorinators. Application of the BS-BA strategy with Dhc as the augmenting organism resulted in complete dechlorination by week 17 with approximately twofold to threefold increase in 16S rRNA and Dhc gene abundance. Live/dead cell counts (LDCC) showed 70–80 % viability in both treatments indicating active growth of potential dechlorinators. The LDCC was strongly correlated with cell copy numbers (r > 0.95) suggesting its potential use for low-cost monitoring of bioremediation. This study also shows the dechlorinating potential of indigenous non-Dhc groups can be successfully exploited for PCE decontamination while demonstrating the applicability of microbiological and chemical methodologies for preliminary site assessments prior to field-based studies.

LanguageEnglish
Pages1869-1880
Number of pages12
JournalInternational Journal of Environmental Science and Technology
Volume11
Issue number7
DOIs
Publication statusPublished - 1 Sep 2014
Externally publishedYes

Keywords

  • Bioaugmentation
  • Biostimulation
  • Cell viability
  • Chlorinated compounds
  • Quantitative PCR

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Environmental Chemistry
  • Environmental Engineering

Cite this

Patil, S. S. ; Adetutu, E. M. ; Sheppard, P. J. ; Morrison, P. ; Menz, I. R. ; Ball, A. S. / Site-specific pre-evaluation of bioremediation technologies for chloroethene degradation. In: International Journal of Environmental Science and Technology. 2014 ; Vol. 11, No. 7. pp. 1869-1880.
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abstract = "Groundwater systems are important sources of water for drinking and irrigation purposes. Unfortunately, human activities have led to widespread groundwater contamination by chlorinated compounds such as tetrachloroethene (PCE). Chloroethenes are extremely harmful to humans and the environment due to their carcinogenic properties. Therefore, this study investigated the potential for bioremediating PCE-contaminated groundwater using laboratory-based biostimulation (BS) and biostimulation–bioaugmentation (BS-BA) assays. This was carried out on groundwater samples obtained from a PCE-contaminated site which had been unsuccessfully treated using chemical oxidation. BS resulted in complete dechlorination by week 21 compared to controls which had only 30 {\%} PCE degradation. BS also led to a approximately threefold increase in 16S rRNA gene copies compared to the controls. However, the major bacterial dechlorinating group, Dehalococcoides (Dhc), was undetectable in PCE-contaminated groundwater. This suggested that dechlorination in BS samples was due to indigenous non-Dhc dechlorinators. Application of the BS-BA strategy with Dhc as the augmenting organism resulted in complete dechlorination by week 17 with approximately twofold to threefold increase in 16S rRNA and Dhc gene abundance. Live/dead cell counts (LDCC) showed 70–80 {\%} viability in both treatments indicating active growth of potential dechlorinators. The LDCC was strongly correlated with cell copy numbers (r > 0.95) suggesting its potential use for low-cost monitoring of bioremediation. This study also shows the dechlorinating potential of indigenous non-Dhc groups can be successfully exploited for PCE decontamination while demonstrating the applicability of microbiological and chemical methodologies for preliminary site assessments prior to field-based studies.",
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Site-specific pre-evaluation of bioremediation technologies for chloroethene degradation. / Patil, S. S.; Adetutu, E. M.; Sheppard, P. J.; Morrison, P.; Menz, I. R.; Ball, A. S.

In: International Journal of Environmental Science and Technology, Vol. 11, No. 7, 01.09.2014, p. 1869-1880.

Research output: Contribution to journalArticle

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