Simultaneous conduction mapping and intracellular membrane potential recording in isolated atria

Melissa Neo, David G. Morris, Pawel Kuklik, Dennis H. Lau, Hany Dimitri, Wei Wen Lim, Prashanthan Sanders, David A. Saint

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

We describe a novel approach for simultaneously determining regional differences in action potential (AP) morphology and tissue electrophysiological properties in isolated atria. The epicardial surface of rat atrial preparations was placed in contact with a multi-electrode array (9 × 10 silver chloride electrodes, 0.1mmdiameter and 0.1mmpitch). A glass microelectrode (100M[1]) was simultaneously inserted into the endocardial surface to record intracellular AP from either of 2 regions (A, B) during pacing from 2 opposite corners of the tissue. AP duration at 80% of repolarisation and its restitution curve was significantly different only in region A (p < 0.01) when AP was initiated at different stimulation sites. Alternans in AP duration and AP amplitude, and in conduction velocity were observed during 2 separate arrhythmic episodes. This approach of combining microelectrode array and intracellular membrane potential recording may provide new insights into arrhythmogenic mechanisms in animal models of cardiovascular disease.

Original languageEnglish
Pages (from-to)563-569
Number of pages7
JournalCanadian journal of physiology and pharmacology
Volume94
Issue number5
DOIs
Publication statusPublished or Issued - 2016

Keywords

  • Arrhythmogenic mechanisms
  • Atrial action potential membrane recordings
  • Atrial fibrillation
  • Electrophysiology
  • High-density mapping

ASJC Scopus subject areas

  • Physiology
  • Pharmacology
  • Physiology (medical)

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