Simple HPLC method for determination of rosiglitazone in sheep plasma and amniotic fluid and its application in a pregnant sheep model

Maryam Bazargan, Andrew K. Davey, Beverly S. Muhlhausler, Janna L. Morrison, I. Caroline McMillen, David J R Foster

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

This paper describes the development of a sensitive high performance liquid chromatography (HPLC) method for quantification of rosiglitazone in sheep plasma and amniotic fluid. Samples were prepared by liquid-liquid extraction using tert-butyl methyl ether, and rosiglitazone was quantitated by HPLC using a C18 column and fluorescence detector with an excitation wavelength of 247. nm and emission wavelength of 367. nm. The mobile phase consisted of ammonium acetate (10. mM, pH 5.2) and acetonitrile (56.5:43.5, v/v) with a flow rate of 1. ml/min. Ketoconazole was used as the internal standard (IS).The plasma calibration curve was linear over the range of 2.5-250ng/ml (mean r2=0.9940±0.0024; n=6) with accuracy of 99.4-102.8% over the calibration range. The intra-day and inter-day coefficient of variation (%CV, percent coefficient of variation) were in the range of 0.01-8.68% in sheep plasma. Similar performance was achieved for amniotic fluid. The described method was successfully applied to quantitate rosiglitazone concentrations in the pregnant ewe and her fetus.

LanguageEnglish
Pages360-365
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume55
Issue number2
DOIs
Publication statusPublished - 15 May 2011
Externally publishedYes

Keywords

  • Fetus
  • HPLC
  • Placenta
  • Pregnant sheep
  • Rosiglitazone

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

Cite this

Bazargan, Maryam ; Davey, Andrew K. ; Muhlhausler, Beverly S. ; Morrison, Janna L. ; McMillen, I. Caroline ; Foster, David J R. / Simple HPLC method for determination of rosiglitazone in sheep plasma and amniotic fluid and its application in a pregnant sheep model. In: Journal of Pharmaceutical and Biomedical Analysis. 2011 ; Vol. 55, No. 2. pp. 360-365.
@article{64a0a9ebe8e34f04978d363368a1617e,
title = "Simple HPLC method for determination of rosiglitazone in sheep plasma and amniotic fluid and its application in a pregnant sheep model",
abstract = "This paper describes the development of a sensitive high performance liquid chromatography (HPLC) method for quantification of rosiglitazone in sheep plasma and amniotic fluid. Samples were prepared by liquid-liquid extraction using tert-butyl methyl ether, and rosiglitazone was quantitated by HPLC using a C18 column and fluorescence detector with an excitation wavelength of 247. nm and emission wavelength of 367. nm. The mobile phase consisted of ammonium acetate (10. mM, pH 5.2) and acetonitrile (56.5:43.5, v/v) with a flow rate of 1. ml/min. Ketoconazole was used as the internal standard (IS).The plasma calibration curve was linear over the range of 2.5-250ng/ml (mean r2=0.9940±0.0024; n=6) with accuracy of 99.4-102.8{\%} over the calibration range. The intra-day and inter-day coefficient of variation ({\%}CV, percent coefficient of variation) were in the range of 0.01-8.68{\%} in sheep plasma. Similar performance was achieved for amniotic fluid. The described method was successfully applied to quantitate rosiglitazone concentrations in the pregnant ewe and her fetus.",
keywords = "Fetus, HPLC, Placenta, Pregnant sheep, Rosiglitazone",
author = "Maryam Bazargan and Davey, {Andrew K.} and Muhlhausler, {Beverly S.} and Morrison, {Janna L.} and McMillen, {I. Caroline} and Foster, {David J R}",
year = "2011",
month = "5",
day = "15",
doi = "10.1016/j.jpba.2011.01.026",
language = "English",
volume = "55",
pages = "360--365",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
issn = "0731-7085",
publisher = "Elsevier",
number = "2",

}

Simple HPLC method for determination of rosiglitazone in sheep plasma and amniotic fluid and its application in a pregnant sheep model. / Bazargan, Maryam; Davey, Andrew K.; Muhlhausler, Beverly S.; Morrison, Janna L.; McMillen, I. Caroline; Foster, David J R.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 55, No. 2, 15.05.2011, p. 360-365.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Simple HPLC method for determination of rosiglitazone in sheep plasma and amniotic fluid and its application in a pregnant sheep model

AU - Bazargan, Maryam

AU - Davey, Andrew K.

AU - Muhlhausler, Beverly S.

AU - Morrison, Janna L.

AU - McMillen, I. Caroline

AU - Foster, David J R

PY - 2011/5/15

Y1 - 2011/5/15

N2 - This paper describes the development of a sensitive high performance liquid chromatography (HPLC) method for quantification of rosiglitazone in sheep plasma and amniotic fluid. Samples were prepared by liquid-liquid extraction using tert-butyl methyl ether, and rosiglitazone was quantitated by HPLC using a C18 column and fluorescence detector with an excitation wavelength of 247. nm and emission wavelength of 367. nm. The mobile phase consisted of ammonium acetate (10. mM, pH 5.2) and acetonitrile (56.5:43.5, v/v) with a flow rate of 1. ml/min. Ketoconazole was used as the internal standard (IS).The plasma calibration curve was linear over the range of 2.5-250ng/ml (mean r2=0.9940±0.0024; n=6) with accuracy of 99.4-102.8% over the calibration range. The intra-day and inter-day coefficient of variation (%CV, percent coefficient of variation) were in the range of 0.01-8.68% in sheep plasma. Similar performance was achieved for amniotic fluid. The described method was successfully applied to quantitate rosiglitazone concentrations in the pregnant ewe and her fetus.

AB - This paper describes the development of a sensitive high performance liquid chromatography (HPLC) method for quantification of rosiglitazone in sheep plasma and amniotic fluid. Samples were prepared by liquid-liquid extraction using tert-butyl methyl ether, and rosiglitazone was quantitated by HPLC using a C18 column and fluorescence detector with an excitation wavelength of 247. nm and emission wavelength of 367. nm. The mobile phase consisted of ammonium acetate (10. mM, pH 5.2) and acetonitrile (56.5:43.5, v/v) with a flow rate of 1. ml/min. Ketoconazole was used as the internal standard (IS).The plasma calibration curve was linear over the range of 2.5-250ng/ml (mean r2=0.9940±0.0024; n=6) with accuracy of 99.4-102.8% over the calibration range. The intra-day and inter-day coefficient of variation (%CV, percent coefficient of variation) were in the range of 0.01-8.68% in sheep plasma. Similar performance was achieved for amniotic fluid. The described method was successfully applied to quantitate rosiglitazone concentrations in the pregnant ewe and her fetus.

KW - Fetus

KW - HPLC

KW - Placenta

KW - Pregnant sheep

KW - Rosiglitazone

UR - http://www.scopus.com/inward/record.url?scp=79952247295&partnerID=8YFLogxK

U2 - 10.1016/j.jpba.2011.01.026

DO - 10.1016/j.jpba.2011.01.026

M3 - Article

VL - 55

SP - 360

EP - 365

JO - Journal of Pharmaceutical and Biomedical Analysis

T2 - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

SN - 0731-7085

IS - 2

ER -