Short Cationic Peptide Derived from Archaea with Dual Antibacterial Properties and Anti-Infective Potential

Elizabete S. Cândido, Marlon H. Cardoso, Lai Y. Chan, Marcelo D.T. Torres, Karen G.N. Oshiro, William F. Porto, Suzana M. Ribeiro, Evan F. Haney, Robert E.W. Hancock, Timothy K. Lu, Cesar De La Fuente-Nunez, David J. Craik, Octávio L. Franco

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Bacterial biofilms and associated infections represent one of the biggest challenges in the clinic, and as an alternative to counter bacterial infections, antimicrobial peptides have attracted great attention in the past decade. Here, ten short cationic antimicrobial peptides were generated through a sliding-window strategy on the basis of the 19-amino acid residue peptide, derived from a Pyrobaculum aerophilum ribosomal protein. PaDBS1R6F10 exhibited anti-infective potential as it decreased the bacterial burden in murine Pseudomonas aeruginosa cutaneous infections by more than 1000-fold. Adverse cytotoxic and hemolytic effects were not detected against mammalian cells. The peptide demonstrated structural plasticity in terms of its secondary structure in the different environments tested. PaDBS1R6F10 represents a promising antimicrobial agent against bacteria infections, without harming human cells.

Original languageEnglish
Pages (from-to)1081-1086
Number of pages6
JournalACS Infectious Diseases
Volume5
Issue number7
DOIs
Publication statusPublished - 24 Apr 2019
Externally publishedYes

Keywords

  • CD spectroscopy
  • antimicrobial peptide
  • biofilm
  • cutaneous infection

ASJC Scopus subject areas

  • Infectious Diseases

Cite this