SHED: Stem cells from human exfoliated deciduous teeth

Masako Miura, Stan Gronthos, Mingrui Zhao, Bai Lu, Larry W. Fisher, Pamela Gehron Robey, Songtao Shi

Research output: Contribution to journalArticlepeer-review

1907 Citations (Scopus)

Abstract

To isolate high-quality human postnatal stem cells from accessible resources is an important goal for stem-cell research. In this study we found that exfoliated human deciduous tooth contains multipotent stem cells [stem cells from human exfoliated deciduous teeth (SHED)]. SHED were identified to be a population of highly proliferative, clonogenic cells capable of differentiating into a variety of cell types including neural cells, adipocytes, and odontoblasts. After in vivo transplantation, SHED were found to be able to induce bone formation, generate dentin, and survive in mouse brain along with expression of neural markers. Here we show that a naturally exfoliated human organ contains a population of stem cells that are completely different from previously identified stem cells. SHED are not only derived from a very accessible tissue resource but are also capable of providing enough cells for potential clinical application. Thus, exfoliated teeth may be an unexpected unique resource for stem-cell therapies including autologous stem-cell transplantation and tissue engineering.

Original languageEnglish
Pages (from-to)5807-5812
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number10
DOIs
Publication statusPublished or Issued - 13 May 2003

Keywords

  • Adipocyte
  • Bone regeneration
  • Dental pulp stem cell
  • Neural differentiation
  • Odontoblast

ASJC Scopus subject areas

  • General

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