Serum sex steroids and steroidogenesis-related enzyme expression in skeletal muscle during experimental weight gain in men

K. Sato, D. Samocha-Bonet, D. J. Handelsman, S. Fujita, G. A. Wittert, L. K. Heilbronn

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Low-circulating testosterone is associated with development of type 2 diabetes in obese men. In this study, we examined the effects of experimental overfeeding and weight gain on serum levels of sex hormones and skeletal muscle expression of steroidogenic enzymes in healthy men with (FH+) and without (FH-) a family history of type 2 diabetes. Methods: Following a 3-day lead in energy balanced diet, FH+ (. n=. 9) and FH- men (. n=. 11) were overfed by 5200. kJ/day (45% fat) for 28. days. Body weight, fasting glucose, insulin, sex steroid, sex hormone binding globulin (SHBG) levels, insulin sensitivity (hyperinsulinaemic-euglycaemic clamp) and body fat (DXA) were assessed in all individuals at baseline and day. 28, and sex steroidogenesis-related enzyme expression in vastus lateralis biopsies was examined in a subset (. n=. 11). Results: Body weight, fat mass and fasting insulin levels were increased by overfeeding (. P<. 0.01) and insulin was increased significantly more in FH+ men (. P<. 0.01). Serum sex hormone binding globulin (SHBG) and 5α-dihydrotestosterone (DHT) were reduced with overfeeding (. P<. 0.05), and serum testosterone and DHT were reduced to a greater extent in FH+ men (. P<. 0.05). Overfeeding reduced mRNA expression of 3β-hydroxysteroid dehydrogenase (HSD) and 17βHSD (. P≤. 0.007), independently of group. 5α-Reductase (SRD5A1) mRNA expression was not changed overall, but a time by group interaction was observed (. P=. 0.04). Conclusion: Overfeeding reduced SHBG and muscle expression of enzymes involved in the formation of testosterone in skeletal muscle. Men with a family history of T2DM were more susceptible to deleterious outcomes of overfeeding with greater reductions in serum testosterone and DHT and greater increases in markers of insulin resistance, which may contribute to increased risk of developing type 2 diabetes.

LanguageEnglish
Pages439-444
Number of pages6
JournalDiabetes and Metabolism
Volume40
Issue number6
DOIs
Publication statusPublished - 1 Jan 2014

Keywords

  • Insulin resistance
  • Overfeeding, Serum testosterone
  • Steroidogenic enzymes

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

@article{12cd3e8ccebe482f96e93ee745db435c,
title = "Serum sex steroids and steroidogenesis-related enzyme expression in skeletal muscle during experimental weight gain in men",
abstract = "Low-circulating testosterone is associated with development of type 2 diabetes in obese men. In this study, we examined the effects of experimental overfeeding and weight gain on serum levels of sex hormones and skeletal muscle expression of steroidogenic enzymes in healthy men with (FH+) and without (FH-) a family history of type 2 diabetes. Methods: Following a 3-day lead in energy balanced diet, FH+ (. n=. 9) and FH- men (. n=. 11) were overfed by 5200. kJ/day (45{\%} fat) for 28. days. Body weight, fasting glucose, insulin, sex steroid, sex hormone binding globulin (SHBG) levels, insulin sensitivity (hyperinsulinaemic-euglycaemic clamp) and body fat (DXA) were assessed in all individuals at baseline and day. 28, and sex steroidogenesis-related enzyme expression in vastus lateralis biopsies was examined in a subset (. n=. 11). Results: Body weight, fat mass and fasting insulin levels were increased by overfeeding (. P<. 0.01) and insulin was increased significantly more in FH+ men (. P<. 0.01). Serum sex hormone binding globulin (SHBG) and 5α-dihydrotestosterone (DHT) were reduced with overfeeding (. P<. 0.05), and serum testosterone and DHT were reduced to a greater extent in FH+ men (. P<. 0.05). Overfeeding reduced mRNA expression of 3β-hydroxysteroid dehydrogenase (HSD) and 17βHSD (. P≤. 0.007), independently of group. 5α-Reductase (SRD5A1) mRNA expression was not changed overall, but a time by group interaction was observed (. P=. 0.04). Conclusion: Overfeeding reduced SHBG and muscle expression of enzymes involved in the formation of testosterone in skeletal muscle. Men with a family history of T2DM were more susceptible to deleterious outcomes of overfeeding with greater reductions in serum testosterone and DHT and greater increases in markers of insulin resistance, which may contribute to increased risk of developing type 2 diabetes.",
keywords = "Insulin resistance, Overfeeding, Serum testosterone, Steroidogenic enzymes",
author = "K. Sato and D. Samocha-Bonet and Handelsman, {D. J.} and S. Fujita and Wittert, {G. A.} and Heilbronn, {L. K.}",
year = "2014",
month = "1",
day = "1",
doi = "10.1016/j.diabet.2014.03.006",
language = "English",
volume = "40",
pages = "439--444",
journal = "Diabetes and Metabolism",
issn = "1262-3636",
publisher = "Elsevier Masson",
number = "6",

}

Serum sex steroids and steroidogenesis-related enzyme expression in skeletal muscle during experimental weight gain in men. / Sato, K.; Samocha-Bonet, D.; Handelsman, D. J.; Fujita, S.; Wittert, G. A.; Heilbronn, L. K.

In: Diabetes and Metabolism, Vol. 40, No. 6, 01.01.2014, p. 439-444.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Serum sex steroids and steroidogenesis-related enzyme expression in skeletal muscle during experimental weight gain in men

AU - Sato, K.

AU - Samocha-Bonet, D.

AU - Handelsman, D. J.

AU - Fujita, S.

AU - Wittert, G. A.

AU - Heilbronn, L. K.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Low-circulating testosterone is associated with development of type 2 diabetes in obese men. In this study, we examined the effects of experimental overfeeding and weight gain on serum levels of sex hormones and skeletal muscle expression of steroidogenic enzymes in healthy men with (FH+) and without (FH-) a family history of type 2 diabetes. Methods: Following a 3-day lead in energy balanced diet, FH+ (. n=. 9) and FH- men (. n=. 11) were overfed by 5200. kJ/day (45% fat) for 28. days. Body weight, fasting glucose, insulin, sex steroid, sex hormone binding globulin (SHBG) levels, insulin sensitivity (hyperinsulinaemic-euglycaemic clamp) and body fat (DXA) were assessed in all individuals at baseline and day. 28, and sex steroidogenesis-related enzyme expression in vastus lateralis biopsies was examined in a subset (. n=. 11). Results: Body weight, fat mass and fasting insulin levels were increased by overfeeding (. P<. 0.01) and insulin was increased significantly more in FH+ men (. P<. 0.01). Serum sex hormone binding globulin (SHBG) and 5α-dihydrotestosterone (DHT) were reduced with overfeeding (. P<. 0.05), and serum testosterone and DHT were reduced to a greater extent in FH+ men (. P<. 0.05). Overfeeding reduced mRNA expression of 3β-hydroxysteroid dehydrogenase (HSD) and 17βHSD (. P≤. 0.007), independently of group. 5α-Reductase (SRD5A1) mRNA expression was not changed overall, but a time by group interaction was observed (. P=. 0.04). Conclusion: Overfeeding reduced SHBG and muscle expression of enzymes involved in the formation of testosterone in skeletal muscle. Men with a family history of T2DM were more susceptible to deleterious outcomes of overfeeding with greater reductions in serum testosterone and DHT and greater increases in markers of insulin resistance, which may contribute to increased risk of developing type 2 diabetes.

AB - Low-circulating testosterone is associated with development of type 2 diabetes in obese men. In this study, we examined the effects of experimental overfeeding and weight gain on serum levels of sex hormones and skeletal muscle expression of steroidogenic enzymes in healthy men with (FH+) and without (FH-) a family history of type 2 diabetes. Methods: Following a 3-day lead in energy balanced diet, FH+ (. n=. 9) and FH- men (. n=. 11) were overfed by 5200. kJ/day (45% fat) for 28. days. Body weight, fasting glucose, insulin, sex steroid, sex hormone binding globulin (SHBG) levels, insulin sensitivity (hyperinsulinaemic-euglycaemic clamp) and body fat (DXA) were assessed in all individuals at baseline and day. 28, and sex steroidogenesis-related enzyme expression in vastus lateralis biopsies was examined in a subset (. n=. 11). Results: Body weight, fat mass and fasting insulin levels were increased by overfeeding (. P<. 0.01) and insulin was increased significantly more in FH+ men (. P<. 0.01). Serum sex hormone binding globulin (SHBG) and 5α-dihydrotestosterone (DHT) were reduced with overfeeding (. P<. 0.05), and serum testosterone and DHT were reduced to a greater extent in FH+ men (. P<. 0.05). Overfeeding reduced mRNA expression of 3β-hydroxysteroid dehydrogenase (HSD) and 17βHSD (. P≤. 0.007), independently of group. 5α-Reductase (SRD5A1) mRNA expression was not changed overall, but a time by group interaction was observed (. P=. 0.04). Conclusion: Overfeeding reduced SHBG and muscle expression of enzymes involved in the formation of testosterone in skeletal muscle. Men with a family history of T2DM were more susceptible to deleterious outcomes of overfeeding with greater reductions in serum testosterone and DHT and greater increases in markers of insulin resistance, which may contribute to increased risk of developing type 2 diabetes.

KW - Insulin resistance

KW - Overfeeding, Serum testosterone

KW - Steroidogenic enzymes

UR - http://www.scopus.com/inward/record.url?scp=84919690408&partnerID=8YFLogxK

U2 - 10.1016/j.diabet.2014.03.006

DO - 10.1016/j.diabet.2014.03.006

M3 - Article

VL - 40

SP - 439

EP - 444

JO - Diabetes and Metabolism

T2 - Diabetes and Metabolism

JF - Diabetes and Metabolism

SN - 1262-3636

IS - 6

ER -