Sequence and relatedness in other bacteria of the Pseudomonas aeruginosa oprP gene coding for the phosphate‐specific porin P

R. Siehnel, N. L. Martin, Robert Hancock

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The oprP gene encoding the Pseudomonas aeruginosa phosphate‐specific outer membrane porin protein OprP was sequenced. Comparison of the derived amino acid sequence with the known sequences of other bacterial porins demonstrated that OprP could be no better aligned to these porin sequences than it could to the periplasmic phosphate‐binding protein PhoS of Escherichia coli. Southern hybridization and restriction mapping of the oprP gene in 37 clinical isolates and the 17 serotype strains of P. aeruginosa revealed that restriction sites in the vicinity of the oprP gene were highly conserved. Several species from the Pseudomonas fluorescens rRNA homology group contained DNA that hybridized to an oprP gene probe.

LanguageEnglish
Pages831-838
Number of pages8
JournalMolecular Microbiology
Volume4
Issue number5
DOIs
Publication statusPublished - 1 Jan 1990
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this