Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells

Naohisa Wada, Hidefumi Maeda, Daigaku Hasegawa, Stan Gronthos, Peter Mark Bartold, Danijela Menicanin, Shinsuke Fujii, Shinichiro Yoshida, Atsushi Tomokiyo, Satoshi Monnouchi, Akifumi Akamine

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Periodontal ligament stem cells (PDLSCs) have recently been proposed as a novel option in periodontal regenerative therapy. However, one of the issues is the difficulty of stably generating PDLSCs because of the variation of stem cell potential between donors. Here, we show that Semaphorin 3A (Sema3A) can induce mesenchymal-stem-like properties in human periodontal ligament (PDL) cells. Sema3A expression was specifically observed in the dental follicle during tooth development and in parts of mature PDL tissue in rodent tooth and periodontal tissue. Sema3A expression levels were found to be higher in multipotential human PDL cell clones compared with low-differentiation potential clones. Sema3A-overexpressing PDL cells exhibited an enhanced capacity to differentiate into both functional osteoblasts and adipocytes. Moreover, PDL cells treated with Sema3A only at the initiation of culture stimulated osteogenesis, while Sema3A treatment throughout the culture had no effect on osteogenic differentiation. Finally, Sema3A-overexpressing PDL cells upregulated the expression of embryonic stem cell markers (NANOG, OCT4, and E-cadherin) and mesenchymal stem cell markers (CD73, CD90, CD105, CD146, and CD166), and Sema3A promoted cell division activity of PDL cells. These results suggest that Sema3A may possess the function to convert PDL cells into mesenchymal-stem-like cells.

Original languageEnglish
Pages (from-to)2225-2236
Number of pages12
JournalStem cells and development
Volume23
Issue number18
DOIs
Publication statusPublished - 15 Sep 2014

ASJC Scopus subject areas

  • Hematology
  • Developmental Biology
  • Cell Biology

Cite this

Wada, N., Maeda, H., Hasegawa, D., Gronthos, S., Bartold, P. M., Menicanin, D., ... Akamine, A. (2014). Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells. Stem cells and development, 23(18), 2225-2236. https://doi.org/10.1089/scd.2013.0405