Secondary acylation of Klebsiella pneumoniae lipopolysaccharide contributes to sensitivity to antibacterial peptides

Abigail Clements, Dedreia Tull, Adam W. Jenney, Jacinta L. Farn, Sang Hyun Kim, Russell E. Bishop, Joseph B. McPhee, Robert Hancock, Elizabeth L. Hartland, Martin J. Pearse, Odilia L.C. Wijburg, David C. Jackson, Malcolm J. McConville, Richard A. Strugnell

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Klebsiella pneumoniae is an important cause of nosocomial Gram-negative sepsis. Lipopolysaccharide (LPS) is considered to be a major virulence determinant of this encapsulated bacterium and most mutations to the lipid A anchor of LPS are conditionally lethal to the bacterium. We studied the role of LPS acylation in K. pneumoniae disease pathogenesis by using a mutation of lpxM (msbB/waaN), which encodes the enzyme responsible for late secondary acylation of immature lipid A molecules. A K. pneumoniae B5055 (K2:O1) lpxM mutant was found to be attenuated for growth in the lungs in a mouse pneumonia model leading to reduced lethality of the bacterium. B5055ΔlpxM exhibited similar sensitivity to phagocytosis or complement-mediated lysis than B5055, unlike the non-encapsulated mutant B5055nm. In vitro, B5055ΔlpxM showed increased permeability of the outer membrane and an increased susceptibility to certain antibacterial peptides suggesting that in vivo attenuation may be due in part to sensitivity to antibacterial peptides present in the lungs of BALB/c mice. These data support the view that lipopolysaccharide acylation plays a important role in providing Gram-negative bacteria some resistance to structural and innate defenses and especially the antibacterial properties of detergents (e.g. bile) and cationic defensins.

LanguageEnglish
Pages15569-15577
Number of pages9
JournalJournal of Biological Chemistry
Volume282
Issue number21
DOIs
Publication statusPublished - 25 May 2007
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Clements, A., Tull, D., Jenney, A. W., Farn, J. L., Kim, S. H., Bishop, R. E., ... Strugnell, R. A. (2007). Secondary acylation of Klebsiella pneumoniae lipopolysaccharide contributes to sensitivity to antibacterial peptides. Journal of Biological Chemistry, 282(21), 15569-15577. https://doi.org/10.1074/jbc.M701454200
Clements, Abigail ; Tull, Dedreia ; Jenney, Adam W. ; Farn, Jacinta L. ; Kim, Sang Hyun ; Bishop, Russell E. ; McPhee, Joseph B. ; Hancock, Robert ; Hartland, Elizabeth L. ; Pearse, Martin J. ; Wijburg, Odilia L.C. ; Jackson, David C. ; McConville, Malcolm J. ; Strugnell, Richard A. / Secondary acylation of Klebsiella pneumoniae lipopolysaccharide contributes to sensitivity to antibacterial peptides. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 21. pp. 15569-15577.
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Clements, A, Tull, D, Jenney, AW, Farn, JL, Kim, SH, Bishop, RE, McPhee, JB, Hancock, R, Hartland, EL, Pearse, MJ, Wijburg, OLC, Jackson, DC, McConville, MJ & Strugnell, RA 2007, 'Secondary acylation of Klebsiella pneumoniae lipopolysaccharide contributes to sensitivity to antibacterial peptides', Journal of Biological Chemistry, vol. 282, no. 21, pp. 15569-15577. https://doi.org/10.1074/jbc.M701454200

Secondary acylation of Klebsiella pneumoniae lipopolysaccharide contributes to sensitivity to antibacterial peptides. / Clements, Abigail; Tull, Dedreia; Jenney, Adam W.; Farn, Jacinta L.; Kim, Sang Hyun; Bishop, Russell E.; McPhee, Joseph B.; Hancock, Robert; Hartland, Elizabeth L.; Pearse, Martin J.; Wijburg, Odilia L.C.; Jackson, David C.; McConville, Malcolm J.; Strugnell, Richard A.

In: Journal of Biological Chemistry, Vol. 282, No. 21, 25.05.2007, p. 15569-15577.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Secondary acylation of Klebsiella pneumoniae lipopolysaccharide contributes to sensitivity to antibacterial peptides

AU - Clements, Abigail

AU - Tull, Dedreia

AU - Jenney, Adam W.

AU - Farn, Jacinta L.

AU - Kim, Sang Hyun

AU - Bishop, Russell E.

AU - McPhee, Joseph B.

AU - Hancock, Robert

AU - Hartland, Elizabeth L.

AU - Pearse, Martin J.

AU - Wijburg, Odilia L.C.

AU - Jackson, David C.

AU - McConville, Malcolm J.

AU - Strugnell, Richard A.

PY - 2007/5/25

Y1 - 2007/5/25

N2 - Klebsiella pneumoniae is an important cause of nosocomial Gram-negative sepsis. Lipopolysaccharide (LPS) is considered to be a major virulence determinant of this encapsulated bacterium and most mutations to the lipid A anchor of LPS are conditionally lethal to the bacterium. We studied the role of LPS acylation in K. pneumoniae disease pathogenesis by using a mutation of lpxM (msbB/waaN), which encodes the enzyme responsible for late secondary acylation of immature lipid A molecules. A K. pneumoniae B5055 (K2:O1) lpxM mutant was found to be attenuated for growth in the lungs in a mouse pneumonia model leading to reduced lethality of the bacterium. B5055ΔlpxM exhibited similar sensitivity to phagocytosis or complement-mediated lysis than B5055, unlike the non-encapsulated mutant B5055nm. In vitro, B5055ΔlpxM showed increased permeability of the outer membrane and an increased susceptibility to certain antibacterial peptides suggesting that in vivo attenuation may be due in part to sensitivity to antibacterial peptides present in the lungs of BALB/c mice. These data support the view that lipopolysaccharide acylation plays a important role in providing Gram-negative bacteria some resistance to structural and innate defenses and especially the antibacterial properties of detergents (e.g. bile) and cationic defensins.

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