Role of Pseudomonas aeruginosa outer membrane protein OprH in polymyxin and gentamicin resistance: Isolation of an OprH-deficient mutant by gene replacement techniques

M. L. Young, M. Bains, A. Bell, R. E.W. Hancock

Research output: Contribution to journalComment/debatepeer-review

48 Citations (Scopus)

Abstract

The cloned oprH gene of Pseudomonas aeruginosa was mutated by inserting a 1.4-kbp fragment that encodes tetracycline resistance. This mutated gene was then incorporated into the P. aeruginosa chromosome by homologous recombination. Growth of the resultant oprH::tet mutant in Mg2+-deficient medium had little effect on susceptibility to polymyxin B, gentamicin, or EDTA unless the mutant was complemented by the cloned oprH gene in plasmid pGB25. In contrast, growth of the parent strain on Mg2+-deficient medium resulted in resistance to all three agents. These data support the hypothesis that overexpression of OprH under Mg2+-deficient growth conditions is conditionally required for resistance to these three agents.

Original languageEnglish
Pages (from-to)2566-2568
Number of pages3
JournalAntimicrobial Agents and Chemotherapy
Volume36
Issue number11
DOIs
Publication statusPublished - 1992
Externally publishedYes

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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