Role of Pseudomonas aeruginosa outer membrane protein OprH in polymyxin and gentamicin resistance: Isolation of an OprH-deficient mutant by gene replacement techniques

M. L. Young, M. Bains, A. Bell, Robert Hancock

Research output: Contribution to journalComment/debate

45 Citations (Scopus)


The cloned oprH gene of Pseudomonas aeruginosa was mutated by inserting a 1.4-kbp fragment that encodes tetracycline resistance. This mutated gene was then incorporated into the P. aeruginosa chromosome by homologous recombination. Growth of the resultant oprH::tet mutant in Mg2+-deficient medium had little effect on susceptibility to polymyxin B, gentamicin, or EDTA unless the mutant was complemented by the cloned oprH gene in plasmid pGB25. In contrast, growth of the parent strain on Mg2+-deficient medium resulted in resistance to all three agents. These data support the hypothesis that overexpression of OprH under Mg2+-deficient growth conditions is conditionally required for resistance to these three agents.

Number of pages3
JournalAntimicrobial Agents and Chemotherapy
Issue number11
Publication statusPublished - 1 Jan 1992
Externally publishedYes

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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