Regulation of Sialidase Production in Clostridium perfringens by the Orphan Sensor Histidine Kinase ReeS

Thomas J. Hiscox, Paul F. Harrison, Anjana Chakravorty, Jocelyn Choo, Kaori Ohtani, Tohru Shimizu, Jackie K. Cheung, Julian I. Rood

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Clostridium perfringens is ubiquitous in nature and is often found as a commensal of the human and animal gastrointestinal tract. It is the primary etiological agent of clostridial myonecrosis, or gas gangrene, a serious infection that results in extensive tissue necrosis due to the action of one or more potent extracellular toxins. α-toxin and perfringolysin O are the major extracellular toxins involved in the pathogenesis of gas gangrene, but histotoxic strains of C. perfringens, such as strain 13, also produce many degradative enzymes such as collagenases, hyaluronidases, sialidases and the cysteine protease, α-clostripain. The production of many of these toxins is regulated either directly or indirectly by the global VirSR two-component signal transduction system. By isolating a chromosomal mutant and carrying out microarray analysis we have identified an orphan sensor histidine kinase, which we have named ReeS (regulator of extracellular enzymes sensor). Expression of the sialidase genes nanI and nanJ was down-regulated in a reeS mutant. Since complementation with the wild-type reeS gene restored nanI and nanJ expression to wild-type levels, as shown by quantitative reverse transcription-PCR and sialidase assays we concluded that ReeS positively regulates the expression of these sialidase genes. However, mutation of the reeS gene had no significant effect on virulence in the mouse myonecrosis model. Sialidase production in C. perfringens has been previously shown to be regulated by both the VirSR system and RevR. In this report, we have analyzed a previously unknown sensor histidine kinase, ReeS, and have shown that it also is involved in controlling the expression of sialidase genes, adding further complexity to the regulatory network that controls sialidase production in C. perfringens.

LanguageEnglish
Article numbere73525
JournalPLoS ONE
Volume8
Issue number9
DOIs
Publication statusPublished - 4 Sep 2013
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Hiscox, T. J., Harrison, P. F., Chakravorty, A., Choo, J., Ohtani, K., Shimizu, T., ... Rood, J. I. (2013). Regulation of Sialidase Production in Clostridium perfringens by the Orphan Sensor Histidine Kinase ReeS. PLoS ONE, 8(9), [e73525]. https://doi.org/10.1371/journal.pone.0073525
Hiscox, Thomas J. ; Harrison, Paul F. ; Chakravorty, Anjana ; Choo, Jocelyn ; Ohtani, Kaori ; Shimizu, Tohru ; Cheung, Jackie K. ; Rood, Julian I. / Regulation of Sialidase Production in Clostridium perfringens by the Orphan Sensor Histidine Kinase ReeS. In: PLoS ONE. 2013 ; Vol. 8, No. 9.
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abstract = "Clostridium perfringens is ubiquitous in nature and is often found as a commensal of the human and animal gastrointestinal tract. It is the primary etiological agent of clostridial myonecrosis, or gas gangrene, a serious infection that results in extensive tissue necrosis due to the action of one or more potent extracellular toxins. α-toxin and perfringolysin O are the major extracellular toxins involved in the pathogenesis of gas gangrene, but histotoxic strains of C. perfringens, such as strain 13, also produce many degradative enzymes such as collagenases, hyaluronidases, sialidases and the cysteine protease, α-clostripain. The production of many of these toxins is regulated either directly or indirectly by the global VirSR two-component signal transduction system. By isolating a chromosomal mutant and carrying out microarray analysis we have identified an orphan sensor histidine kinase, which we have named ReeS (regulator of extracellular enzymes sensor). Expression of the sialidase genes nanI and nanJ was down-regulated in a reeS mutant. Since complementation with the wild-type reeS gene restored nanI and nanJ expression to wild-type levels, as shown by quantitative reverse transcription-PCR and sialidase assays we concluded that ReeS positively regulates the expression of these sialidase genes. However, mutation of the reeS gene had no significant effect on virulence in the mouse myonecrosis model. Sialidase production in C. perfringens has been previously shown to be regulated by both the VirSR system and RevR. In this report, we have analyzed a previously unknown sensor histidine kinase, ReeS, and have shown that it also is involved in controlling the expression of sialidase genes, adding further complexity to the regulatory network that controls sialidase production in C. perfringens.",
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Hiscox, TJ, Harrison, PF, Chakravorty, A, Choo, J, Ohtani, K, Shimizu, T, Cheung, JK & Rood, JI 2013, 'Regulation of Sialidase Production in Clostridium perfringens by the Orphan Sensor Histidine Kinase ReeS', PLoS ONE, vol. 8, no. 9, e73525. https://doi.org/10.1371/journal.pone.0073525

Regulation of Sialidase Production in Clostridium perfringens by the Orphan Sensor Histidine Kinase ReeS. / Hiscox, Thomas J.; Harrison, Paul F.; Chakravorty, Anjana; Choo, Jocelyn; Ohtani, Kaori; Shimizu, Tohru; Cheung, Jackie K.; Rood, Julian I.

In: PLoS ONE, Vol. 8, No. 9, e73525, 04.09.2013.

Research output: Contribution to journalArticle

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AU - Hiscox, Thomas J.

AU - Harrison, Paul F.

AU - Chakravorty, Anjana

AU - Choo, Jocelyn

AU - Ohtani, Kaori

AU - Shimizu, Tohru

AU - Cheung, Jackie K.

AU - Rood, Julian I.

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