Regulation of polypeptide-chain initiation in rat skeletal muscle Starvation does not alter the activity or phosphorylation state of initiation factor eIF-2

Sarah Cox, Nicholas T. Redpath, Christopher G. Proud

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

In rats, 48-h starvation causes a decrease in the rate of protein synthesis in skeletal (e.g. gastrocnemius) muscle, due largely to impairment of peptide-chain initiation. In other cell types inhibition of initiation is associated with decreased activity and recycling of initiation factor eIF-2, and increased phosphorylation of its α-subunit. However, 48-h starvation has no effect on the activity or recycling of eIF-2 measured in extracts of gastrocnemius muscle, or on the level of α-subunit phosphorylation. The effects of starvation on peptide-chain initiation in skeletal muscle must therefore involve alterations in other components of the translational machinery.

Original languageEnglish
Pages (from-to)333-338
Number of pages6
JournalFEBS Letters
Volume239
Issue number2
DOIs
Publication statusPublished or Issued - 7 Nov 1988
Externally publishedYes

Keywords

  • Initiation
  • Initiation factor-2 (eIF-2)
  • Protein phosphorylation
  • Protein synthesis
  • Skeletal muscle

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this