Rationale for the recommendations for harmonizing current methodology for detecting BCR-ABL transcripts in patients with chronic myeloid leukaemia

S. Branford, N. C.P. Cross, A. Hochhaus, J. Radich, G. Saglio, J. Kaeda, J. Goldman, Timothy Hughes

Research output: Contribution to journalReview article

143 Citations (Scopus)

Abstract

Molecular monitoring for patients with chronic myeloid leukaemia (CML) has become an important practice in the era of imatinib therapy. For successful widespread introduction into the mainstream patient monitoring schedule, many procedural aspects of the complex real-time quantitative polymerase chain reaction (RQ-PCR) technique for measuring BCR-ABL transcripts require optimization. Recommendations for harmonizing the differing methodologies have recently been proposed. These recommendations were designed to maximize reliability of analysis for clinical decision making and proposed the adoption of an International Scale of measurement. The purpose of this review is to present the evidence and supporting data for specific recommendations. These recommendations include use of the same source of cells, either blood or marrow, for analysis; for validation of equal PCR amplification efficiencies of cDNA and standards when using a plasmid to construct standard curves and for ensuring ongoing high-level performance by undertaking a quality assurance programme. Clinicians must know the measurement reliability of an RQ-PCR assay to be able to determine the significance of a change in BCR-ABL level. An assay with poor precision limits the clinical usefulness of results. International harmonization should establish RQ-PCR measurement of BCR-ABL as the best method for monitoring treatment response for patients with CML.

LanguageEnglish
Pages1925-1930
Number of pages6
JournalLeukemia
Volume20
Issue number11
DOIs
Publication statusPublished - 1 Jan 2006
Externally publishedYes

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Branford, S. ; Cross, N. C.P. ; Hochhaus, A. ; Radich, J. ; Saglio, G. ; Kaeda, J. ; Goldman, J. ; Hughes, Timothy. / Rationale for the recommendations for harmonizing current methodology for detecting BCR-ABL transcripts in patients with chronic myeloid leukaemia. In: Leukemia. 2006 ; Vol. 20, No. 11. pp. 1925-1930.
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Rationale for the recommendations for harmonizing current methodology for detecting BCR-ABL transcripts in patients with chronic myeloid leukaemia. / Branford, S.; Cross, N. C.P.; Hochhaus, A.; Radich, J.; Saglio, G.; Kaeda, J.; Goldman, J.; Hughes, Timothy.

In: Leukemia, Vol. 20, No. 11, 01.01.2006, p. 1925-1930.

Research output: Contribution to journalReview article

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