Rapid determination of the radiochemical purity of 99mTc- antimony trisulfide colloid prepared by standard and alternative heating methods

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Abstract

The purpose of this study was to validate a rapid quality control method for the lymphoscintigraphy tracer 99mTc-antimony trisulfide colloid (99mTc-ATC). Methods: ATC was labeled with 99mTc according to the manufacturer's instructions as well as by alternative heating conditions designed to provide a range of percentages of radiochemical purity (RCP): the tracer was prepared in a dry block heater with heating cavities of different sizes, the temperature of the heating block was varied from 70°C to 115°C, or the duration of heating was varied from 15 to 35 min. Anion-exchange minicolumns were Mailed to separate any 99mTc- pertechnetate impurity from 99mTc-ATC with physiologic saline as the eluent. Quality control results were compared with the results from the manufacturer's recommended method, which uses an instant thin-layer chromatography (ITLC) strip with saline as the migrating solution. Results: The quality control results obtained with a cartridge method in 2-3 min compared favorably with those obtained with the ITLC method with saline when the tracer was prepared by heating at 115°C in a dry block heater for 35 min (RCPs, 99.4% ± 0.3% [mean ± SD] and 99.2% ± 0.3%, respectively; n = 25). The cartridge and ITLC quality control results also were in excellent agreement (correlation coefficient, 0.99) over a range of RCPs (80%-100%). An alternative anion-exchange cartridge that was tested in this study was not suitable for assaying the RCP of 99mTc-ATC because of the complete retention of 99mTc-pertechnetate on the sorbent. Conclusion: Compared with the established ITLC method, the cartridge quality control method tested in this study is rapid and provides a reliable assessment of the RCP of 99mTc-ATC. For the preparation of 99mTc-ATC, a dry block heater can be successfully substituted for a boiling water bath and is recommended for heating at high altitudes.

Original languageEnglish
Pages (from-to)174-178
Number of pages5
JournalJournal of Nuclear Medicine Technology
Volume34
Issue number3
Publication statusPublished - 1 Dec 2006
Externally publishedYes

Keywords

  • Antimony trisulfide colloid
  • Cartridge
  • Quality control
  • Radiochemical purity
  • Radiocolloid

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

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