Quantitative intravascular biological fluorescence-ultrasound imaging of coronary and peripheral arteries in vivo

Dmitry Bozhko, Eric A. Osborn, Amir Rosenthal, Johan Verjans, Tetsuya Hara, Stephan Kellnberger, Georg Wissmeyer, Saak V. Ovsepian, Jason R. McCarthy, Adam Mauskapf, Ashley F. Stein, Farouc A. Jaffer, Vasilis Ntziachristos

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Aims: (i) to evaluate a novel hybrid near-infrared fluorescence - intravascular ultrasound (NIRF-IVUS) system in coronary and peripheral swine arteries in vivo; (ii) to assess simultaneous quantitative biological and morphological aspects of arterial disease. Methods and results: Two 9F/15MHz peripheral and 4.5F/40MHz coronary near-infrared fluorescence (NIRF)-IVUS catheters were engineered to enable accurate co-registrtation of biological and morphological readings simultaneously in vivo. A correction algorithm utilizing IVUS information was developed to account for the distance-related fluorescence attenuation due to through-blood imaging. Corrected NIRF (cNIRF)-IVUS was applied for in vivo imaging of angioplasty-induced vascular injury in swine peripheral arteries and experimental fibrin deposition on coronary artery stents, and of atheroma in a rabbit aorta, revealing feasibility to intravascularly assay plaque structure and inflammation. The addition of ICG-enhanced NIRF assessment improved the detection of angioplasty-induced endothelial damage compared to standalone IVUS. In addition, NIRF detection of coronary stent fibrin by in vivo cNIRF-IVUS imaging illuminated stent pathobiology that was concealed on standalone IVUS. Fluorescence reflectance imaging and microscopy of resected tissues corroborated the in vivo findings. Conclusions: Integrated cNIRF-IVUS enables simultaneous co-registered through-blood imaging of disease related morphological and biological alterations in coronary and peripheral arteries in vivo. Clinical translation of cNIRF-IVUS may significantly enhance knowledge of arterial pathobiology, leading to improvements in clinical diagnosis and prognosis, and helps to guide the development of new therapeutic approaches for arterial diseases.

LanguageEnglish
Pages1253-1261
Number of pages9
JournalEuropean Heart Journal Cardiovascular Imaging
Volume18
Issue number11
DOIs
Publication statusPublished - 1 Nov 2017
Externally publishedYes

Keywords

  • IVUS
  • NIRF
  • fluorescence imaging
  • molecular imaging

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Cardiology and Cardiovascular Medicine

Cite this

Bozhko, Dmitry ; Osborn, Eric A. ; Rosenthal, Amir ; Verjans, Johan ; Hara, Tetsuya ; Kellnberger, Stephan ; Wissmeyer, Georg ; Ovsepian, Saak V. ; McCarthy, Jason R. ; Mauskapf, Adam ; Stein, Ashley F. ; Jaffer, Farouc A. ; Ntziachristos, Vasilis. / Quantitative intravascular biological fluorescence-ultrasound imaging of coronary and peripheral arteries in vivo. In: European Heart Journal Cardiovascular Imaging. 2017 ; Vol. 18, No. 11. pp. 1253-1261.
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title = "Quantitative intravascular biological fluorescence-ultrasound imaging of coronary and peripheral arteries in vivo",
abstract = "Aims: (i) to evaluate a novel hybrid near-infrared fluorescence - intravascular ultrasound (NIRF-IVUS) system in coronary and peripheral swine arteries in vivo; (ii) to assess simultaneous quantitative biological and morphological aspects of arterial disease. Methods and results: Two 9F/15MHz peripheral and 4.5F/40MHz coronary near-infrared fluorescence (NIRF)-IVUS catheters were engineered to enable accurate co-registrtation of biological and morphological readings simultaneously in vivo. A correction algorithm utilizing IVUS information was developed to account for the distance-related fluorescence attenuation due to through-blood imaging. Corrected NIRF (cNIRF)-IVUS was applied for in vivo imaging of angioplasty-induced vascular injury in swine peripheral arteries and experimental fibrin deposition on coronary artery stents, and of atheroma in a rabbit aorta, revealing feasibility to intravascularly assay plaque structure and inflammation. The addition of ICG-enhanced NIRF assessment improved the detection of angioplasty-induced endothelial damage compared to standalone IVUS. In addition, NIRF detection of coronary stent fibrin by in vivo cNIRF-IVUS imaging illuminated stent pathobiology that was concealed on standalone IVUS. Fluorescence reflectance imaging and microscopy of resected tissues corroborated the in vivo findings. Conclusions: Integrated cNIRF-IVUS enables simultaneous co-registered through-blood imaging of disease related morphological and biological alterations in coronary and peripheral arteries in vivo. Clinical translation of cNIRF-IVUS may significantly enhance knowledge of arterial pathobiology, leading to improvements in clinical diagnosis and prognosis, and helps to guide the development of new therapeutic approaches for arterial diseases.",
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author = "Dmitry Bozhko and Osborn, {Eric A.} and Amir Rosenthal and Johan Verjans and Tetsuya Hara and Stephan Kellnberger and Georg Wissmeyer and Ovsepian, {Saak V.} and McCarthy, {Jason R.} and Adam Mauskapf and Stein, {Ashley F.} and Jaffer, {Farouc A.} and Vasilis Ntziachristos",
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Bozhko, D, Osborn, EA, Rosenthal, A, Verjans, J, Hara, T, Kellnberger, S, Wissmeyer, G, Ovsepian, SV, McCarthy, JR, Mauskapf, A, Stein, AF, Jaffer, FA & Ntziachristos, V 2017, 'Quantitative intravascular biological fluorescence-ultrasound imaging of coronary and peripheral arteries in vivo', European Heart Journal Cardiovascular Imaging, vol. 18, no. 11, pp. 1253-1261. https://doi.org/10.1093/ehjci/jew222

Quantitative intravascular biological fluorescence-ultrasound imaging of coronary and peripheral arteries in vivo. / Bozhko, Dmitry; Osborn, Eric A.; Rosenthal, Amir; Verjans, Johan; Hara, Tetsuya; Kellnberger, Stephan; Wissmeyer, Georg; Ovsepian, Saak V.; McCarthy, Jason R.; Mauskapf, Adam; Stein, Ashley F.; Jaffer, Farouc A.; Ntziachristos, Vasilis.

In: European Heart Journal Cardiovascular Imaging, Vol. 18, No. 11, 01.11.2017, p. 1253-1261.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Quantitative intravascular biological fluorescence-ultrasound imaging of coronary and peripheral arteries in vivo

AU - Bozhko, Dmitry

AU - Osborn, Eric A.

AU - Rosenthal, Amir

AU - Verjans, Johan

AU - Hara, Tetsuya

AU - Kellnberger, Stephan

AU - Wissmeyer, Georg

AU - Ovsepian, Saak V.

AU - McCarthy, Jason R.

AU - Mauskapf, Adam

AU - Stein, Ashley F.

AU - Jaffer, Farouc A.

AU - Ntziachristos, Vasilis

PY - 2017/11/1

Y1 - 2017/11/1

N2 - Aims: (i) to evaluate a novel hybrid near-infrared fluorescence - intravascular ultrasound (NIRF-IVUS) system in coronary and peripheral swine arteries in vivo; (ii) to assess simultaneous quantitative biological and morphological aspects of arterial disease. Methods and results: Two 9F/15MHz peripheral and 4.5F/40MHz coronary near-infrared fluorescence (NIRF)-IVUS catheters were engineered to enable accurate co-registrtation of biological and morphological readings simultaneously in vivo. A correction algorithm utilizing IVUS information was developed to account for the distance-related fluorescence attenuation due to through-blood imaging. Corrected NIRF (cNIRF)-IVUS was applied for in vivo imaging of angioplasty-induced vascular injury in swine peripheral arteries and experimental fibrin deposition on coronary artery stents, and of atheroma in a rabbit aorta, revealing feasibility to intravascularly assay plaque structure and inflammation. The addition of ICG-enhanced NIRF assessment improved the detection of angioplasty-induced endothelial damage compared to standalone IVUS. In addition, NIRF detection of coronary stent fibrin by in vivo cNIRF-IVUS imaging illuminated stent pathobiology that was concealed on standalone IVUS. Fluorescence reflectance imaging and microscopy of resected tissues corroborated the in vivo findings. Conclusions: Integrated cNIRF-IVUS enables simultaneous co-registered through-blood imaging of disease related morphological and biological alterations in coronary and peripheral arteries in vivo. Clinical translation of cNIRF-IVUS may significantly enhance knowledge of arterial pathobiology, leading to improvements in clinical diagnosis and prognosis, and helps to guide the development of new therapeutic approaches for arterial diseases.

AB - Aims: (i) to evaluate a novel hybrid near-infrared fluorescence - intravascular ultrasound (NIRF-IVUS) system in coronary and peripheral swine arteries in vivo; (ii) to assess simultaneous quantitative biological and morphological aspects of arterial disease. Methods and results: Two 9F/15MHz peripheral and 4.5F/40MHz coronary near-infrared fluorescence (NIRF)-IVUS catheters were engineered to enable accurate co-registrtation of biological and morphological readings simultaneously in vivo. A correction algorithm utilizing IVUS information was developed to account for the distance-related fluorescence attenuation due to through-blood imaging. Corrected NIRF (cNIRF)-IVUS was applied for in vivo imaging of angioplasty-induced vascular injury in swine peripheral arteries and experimental fibrin deposition on coronary artery stents, and of atheroma in a rabbit aorta, revealing feasibility to intravascularly assay plaque structure and inflammation. The addition of ICG-enhanced NIRF assessment improved the detection of angioplasty-induced endothelial damage compared to standalone IVUS. In addition, NIRF detection of coronary stent fibrin by in vivo cNIRF-IVUS imaging illuminated stent pathobiology that was concealed on standalone IVUS. Fluorescence reflectance imaging and microscopy of resected tissues corroborated the in vivo findings. Conclusions: Integrated cNIRF-IVUS enables simultaneous co-registered through-blood imaging of disease related morphological and biological alterations in coronary and peripheral arteries in vivo. Clinical translation of cNIRF-IVUS may significantly enhance knowledge of arterial pathobiology, leading to improvements in clinical diagnosis and prognosis, and helps to guide the development of new therapeutic approaches for arterial diseases.

KW - IVUS

KW - NIRF

KW - fluorescence imaging

KW - molecular imaging

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U2 - 10.1093/ehjci/jew222

DO - 10.1093/ehjci/jew222

M3 - Article

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JO - European heart journal cardiovascular Imaging

T2 - European heart journal cardiovascular Imaging

JF - European heart journal cardiovascular Imaging

SN - 2047-2404

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ER -