PVP formulated fullerene (C60) increases Rho-kinase dependent vascular tissue contractility in pregnant Sprague Dawley rats

Achini K. Vidanapathirana, Leslie C. Thompson, Erin E. Mann, Jillian T. Odom, Nathan A. Holland, Susan J. Sumner, Li Han, Anita H. Lewin, Timothy R. Fennell, Jared M. Brown, Christopher J. Wingard

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Pregnancy is a unique physiological state, in which C60 fullerene is reported to be distributed in both maternal and fetal tissues. Tissue distribution of C60 differs between pregnant and non-pregnant states, presumably due to functional changes in vasculature during pregnancy. We hypothesized that polyvinylpyrrolidone (PVP) formulated C60 (C60/PVP) increases vascular tissue contractility during pregnancy by increasing Rho-kinase activity. C60/PVP was administered intravenously to pregnant and non-pregnant female Sprague Dawley rats. Vascular responses were assessed using wire myography 24. h post-exposure. Increased stress generation was observed in uterine artery, thoracic aorta and umbilical vein. Rho-Rho-kinase mediated force maintenance was increased in arterial segments from C60/PVP exposed pregnant rats when compared to PVP exposed rats. Our findings suggest that intravenous exposure to C60/PVP during pregnancy increases vascular tissue contractility of the uterine artery through elements of Rho-Rho-kinase signaling during late stages of pregnancy.

Original languageEnglish
Pages (from-to)86-100
Number of pages15
JournalReproductive Toxicology
Volume49
DOIs
Publication statusPublished or Issued - Nov 2014

Keywords

  • Nanotoxicology
  • Polyvinylpyrrolidone
  • Pregnancy
  • Rho-kinase pathway
  • Umbilical vein
  • Uterine artery
  • Vascular tissue contractility

ASJC Scopus subject areas

  • Toxicology

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