PSGL-1-mediated adhesion of human hematopoietic progenitors to P- selectin results in suppression of hematopoiesis

Jean Pierre Lévesque, Andrew C W Zannettino, Melanie Pudney, Silvana Niutta, David N. Haylock, Karen R. Snapp, Geoffrey S. Kansas, Michael C. Berndt, Paul J. Simmons

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95 Citations (Scopus)

Abstract

Cellular interactions are critical for the regulation of hematopoiesis. The sialomucin PSGL-1/CD162 mediates the attachment of mature leukocytes to P-selectin. We now show that PSGL-1 also functions as the sole receptor for P-selectin on primitive human CD34+ hematopoietic progenitor cells (HPC). More importantly, ligation of PSGL-1 by immobilized or soluble ligand or anti-PSGL-1 antibody results in a profound suppression of HPC proliferation stimulated by potent combinations of early acting hematopoietic growth factors. These data demonstrate an unanticipated but extremely marked growth- inhibitory effect of P-selectin on hematopoiesis and provide direct evidence that PSGL-1, in addition to its well-documented role as an adhesion molecule on mature leukocytes, is a potent negative regulator of human hematopoietic progenitors.

Original languageEnglish
Pages (from-to)369-378
Number of pages10
JournalImmunity
Volume11
Issue number3
DOIs
Publication statusPublished or Issued - Sep 1999

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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