Properties of a Pseudomonas stutzeri outer membrane channel-forming protein (NosA) required for production of copper-containing N2O reductase

H. S. Lee, Robert Hancock, J. L. Ingraham

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Abstract

A protein (NosA) in the outer membrane of Pseudomonas stutzeri that is required for copper to be inserted into N2O reductase has been extracted and purified to homogeneity. The purified protein could form channels in black lipid bilayers. Like N2O reductase, NosA contained copper and was only made anaerobically. In contrast to N2O reductase, its synthesis was repressed by exogenous copper (but not by Mn, Co, Ni, Zn, or Fe). Also in contrast to N2O reductase, NosA homologs were not immunologically detectable in Pseudomonas aeruginosa, Pseudomonas mendocina, Pseudomonas alcaligenes, or other strains of P. stutzeri.

Original languageEnglish
Pages (from-to)2096-2100
Number of pages5
JournalJournal of bacteriology
Volume171
Issue number4
DOIs
Publication statusPublished - 1 Jan 1989
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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