Primitive human hematopoietic progenitors adhere to P-selectin (CD62P)

A. C W Zannettino, M. C. Berndt, C. Butcher, E. C. Butcher, M. A. Vadas, P. J. Simmons

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

P-selectin was shown to bind committed human hematopoietic progenitors (colony-forming unit-granulocyte-macrophage [CFU-GM] and burst-forming unit- erythroid [BFU-E]) as identified by their expression of the CD34 antigen and by in vitro clonogenic assays. In addition, P-selectin bound all precursors (pre-CFU) of committed myeloid progenitors assayed by their ability to sustain hematopoiesis in both conventional stroma-containing and stroma- free, cytokine-dependent systems. Binding of CD34+ cells to P-selectin was temperature-independent and shear-resistant, occurred only in the presence of divalent cations, was protease sensitive, and was completely blocked by anti- P-selectin antibody. Neuraminidase treatment of CD34+ cells completely abrogated their binding to P-selectin, implying a prominent role for sialic acid in the structure and function of the P-selectin ligand on hematopoietic progenitors. Monoclonal antibodies (MoAbs) CSLEX-1 and HECA-452, which identify carbohydrate epitopes involving sialic acid, bound to 33% and 35% of CD34+ cells, respectively, and included the majority of CFU-GM and pre-CFU. Three-color flow cytometric analysis showed a precise codistribution of CSLEX-1 and HECA-452 antigens on CD34+ cells, implying recognition of the same glycoprotein antigen by the two MoAbs. Treatment of CD34+ cells with neuraminidase completely abolished binding of both MoAbs. In addition, HECA- 452 partially blocked the adhesion of CD34+ cells to P-selectin. P-selectin glycoprotein ligand (PSGL-1), recently molecularly cloned from the promyelocytic leukemia cell line HL60, was expressed by CD34+ cells as determined by reverse transcription polymerase chain reaction. Combined with the functional and biochemical characteristics, these data suggest that PSGL- 1 may comprise an important P-selectin ligand expressed by primitive hematopoietic cells, but do not preclude the existence of additional P- selectin ligands on these cells.

LanguageEnglish
Pages3466-3477
Number of pages12
JournalBlood
Volume85
Issue number12
Publication statusPublished - 1 Jan 1995
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Zannettino, A. C. W., Berndt, M. C., Butcher, C., Butcher, E. C., Vadas, M. A., & Simmons, P. J. (1995). Primitive human hematopoietic progenitors adhere to P-selectin (CD62P). Blood, 85(12), 3466-3477.
Zannettino, A. C W ; Berndt, M. C. ; Butcher, C. ; Butcher, E. C. ; Vadas, M. A. ; Simmons, P. J. / Primitive human hematopoietic progenitors adhere to P-selectin (CD62P). In: Blood. 1995 ; Vol. 85, No. 12. pp. 3466-3477.
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abstract = "P-selectin was shown to bind committed human hematopoietic progenitors (colony-forming unit-granulocyte-macrophage [CFU-GM] and burst-forming unit- erythroid [BFU-E]) as identified by their expression of the CD34 antigen and by in vitro clonogenic assays. In addition, P-selectin bound all precursors (pre-CFU) of committed myeloid progenitors assayed by their ability to sustain hematopoiesis in both conventional stroma-containing and stroma- free, cytokine-dependent systems. Binding of CD34+ cells to P-selectin was temperature-independent and shear-resistant, occurred only in the presence of divalent cations, was protease sensitive, and was completely blocked by anti- P-selectin antibody. Neuraminidase treatment of CD34+ cells completely abrogated their binding to P-selectin, implying a prominent role for sialic acid in the structure and function of the P-selectin ligand on hematopoietic progenitors. Monoclonal antibodies (MoAbs) CSLEX-1 and HECA-452, which identify carbohydrate epitopes involving sialic acid, bound to 33{\%} and 35{\%} of CD34+ cells, respectively, and included the majority of CFU-GM and pre-CFU. Three-color flow cytometric analysis showed a precise codistribution of CSLEX-1 and HECA-452 antigens on CD34+ cells, implying recognition of the same glycoprotein antigen by the two MoAbs. Treatment of CD34+ cells with neuraminidase completely abolished binding of both MoAbs. In addition, HECA- 452 partially blocked the adhesion of CD34+ cells to P-selectin. P-selectin glycoprotein ligand (PSGL-1), recently molecularly cloned from the promyelocytic leukemia cell line HL60, was expressed by CD34+ cells as determined by reverse transcription polymerase chain reaction. Combined with the functional and biochemical characteristics, these data suggest that PSGL- 1 may comprise an important P-selectin ligand expressed by primitive hematopoietic cells, but do not preclude the existence of additional P- selectin ligands on these cells.",
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Zannettino, ACW, Berndt, MC, Butcher, C, Butcher, EC, Vadas, MA & Simmons, PJ 1995, 'Primitive human hematopoietic progenitors adhere to P-selectin (CD62P)', Blood, vol. 85, no. 12, pp. 3466-3477.

Primitive human hematopoietic progenitors adhere to P-selectin (CD62P). / Zannettino, A. C W; Berndt, M. C.; Butcher, C.; Butcher, E. C.; Vadas, M. A.; Simmons, P. J.

In: Blood, Vol. 85, No. 12, 01.01.1995, p. 3466-3477.

Research output: Contribution to journalArticle

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Zannettino ACW, Berndt MC, Butcher C, Butcher EC, Vadas MA, Simmons PJ. Primitive human hematopoietic progenitors adhere to P-selectin (CD62P). Blood. 1995 Jan 1;85(12):3466-3477.