Prediction of graft versus host disease by frequency analysis of cytotoxic T cells after unrelated donor bone marrow transplantation

E. Kaminski, J. Hows, S. Man, P. Brookes, S. Mackinnon, T. Hughes, O. Avakian, J. M. Goldman, J. R. Batchelor

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Abstract

HLA 'matched' unrelated donor bone marrow transplants are associated with an increased incidence and severity of graft-versus-host disease in comparison with HLA-identical sibling transplants. This is presumably due to HLA and non-HLA histocompatibility differences between donor and recipient. Using a limiting dilution assay, we have previously demonstrated a relationship between cytotoxic T lymphocyte precursor frequency and HLA disparity. In this study we have compared CTL-p frequencies with clinical GVHD, and demonstrate for the first time a significant correlation (P < 0.005) between high CTL precursor frequency prior to BMT and severity of acute GVHD after HLA A, B, DR 'matched' unrelated donor transplants using T cell depleted marrow. This assay system may be of value in the final selection of HLA 'matched' unrelated donors for BMT.

Original languageEnglish
Pages (from-to)608-613
Number of pages6
JournalTransplantation
Volume48
Issue number4
Publication statusPublished - 1 Jan 1989
Externally publishedYes

ASJC Scopus subject areas

  • Transplantation

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