Potential role of increased matrix metalloproteinase-2 (MMP2) transcription in impaired adipogenesis in type 2 diabetes mellitus

Severine G. Dubois, Yourka D. Tchoukalova, Leonie Heilbronn, Jeanine B. Albu, David E. Kelley, Steven R. Smith, Xiaobing Fang, Eric Ravussin

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

We measured gene expression of paracrine regulators involved in adipocyte differentiation within the stromovascular fraction of abdominal subcutaneous adipose tissue from obese individuals with (n = 30) and without (n = 18) type 2 diabetes mellitus (T2DM). Despite similar adiposity by design, subjects with T2DM had larger adipocytes (0.92 ± 0.28 vs. 0.75 ± 0.17 μl, p < 0.05) than controls. Gene expression of the adipogenic marker aP2 was lower (0.35 ± 0.16 vs. 0.58 ± 0.27 arbitrary units, p < 0.05) whereas the expression of matricellular peptidase, MMP2 was higher (1.65 ± 0.17 vs. 1.27 ± 0.21, p = 0.02) in T2DM vs. controls. The gene expression levels between the aP2 and MMP2 were inversely correlated (r = -0.32, p = 0.03). We conclude that early steps of adipogenesis may be impaired in T2DM independently of obesity due, in part, to an upregulation of the MMP2 transcription.

Original languageEnglish
Pages (from-to)725-728
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume367
Issue number4
DOIs
Publication statusPublished or Issued - 21 Mar 2008
Externally publishedYes

Keywords

  • Adipocyte
  • Fatty acid binding protein aP2
  • Matrix metalloproteinase
  • Obesity
  • Type 2 diabetes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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