A 53-kDa protein from the outer sheath of the oral spirochete Treponema denticola was purified to homogeneity and shown to reconstitute channels in black lipid bilayer model membranes. The channel had a single-channel conductance of 1.8 nS in 0.1 M KCl, making this the largest porin channel observed to date (estimated diameter, 3.4 nm). Electron micrographs of 53- kDa-protein-containing outer sheaths of T. denticola showed a regular hexagonal array of darker staining pits.
|Number of pages||6|
|Journal||Infection and Immunity|
|Publication status||Published or Issued - 1993|
ASJC Scopus subject areas
- Infectious Diseases