Pore-forming properties of the major 53-kilodalton surface antigen from the outer sheath of Treponema denticola

C. Egli, W. K. Leung, K. H. Muller, R. E.W. Hancock, B. C. McBride

Research output: Contribution to journalArticlepeer-review

89 Citations (Scopus)

Abstract

A 53-kDa protein from the outer sheath of the oral spirochete Treponema denticola was purified to homogeneity and shown to reconstitute channels in black lipid bilayer model membranes. The channel had a single-channel conductance of 1.8 nS in 0.1 M KCl, making this the largest porin channel observed to date (estimated diameter, 3.4 nm). Electron micrographs of 53- kDa-protein-containing outer sheaths of T. denticola showed a regular hexagonal array of darker staining pits.

Original languageEnglish
Pages (from-to)1694-1699
Number of pages6
JournalInfection and Immunity
Volume61
Issue number5
Publication statusPublished or Issued - 1993

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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