Platelet rich plasma and dentine effect on sheep dental pulp cells regeneration/revitalization ability (in vitro)

M. Altaii, X. Kaidonis, S. Koblar, P. Cathro, L. Richards

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Platelet rich plasma (PRP) has been proposed as a scaffold for pulp regeneration/revitalization instead of a blood clot. The aim of the following in vitro study was to evaluate the effect of PRP scaffold on proliferation, migration and differentiation of cultured ovine (sheep) dental pulp cells (ODPC) in the presence of dentine. Methods: PRP was prepared by centrifuging blood at 140 g for 12 min. ODPC were cultured on PRP or platelet poor plasma (PPP) scaffolds with and without dentine discs. Cell proliferation, migration and differentiation rates were assessed. Results: ODPC cultured on PRP scaffold showed significantly greater proliferation rates, migration and mineralization compared with cells on PPP or without a scaffold. Dentine discs reduced the proliferation and mineralization potential of the cells. Conclusions: A PRP scaffold has a positive effect on the proliferation, migration and differentiation of ODPC; however, dentine discs have an adverse effect on the activity of ODPC.

LanguageEnglish
Pages39-46
Number of pages8
JournalAustralian Dental Journal
Volume62
Issue number1
DOIs
Publication statusPublished - 1 Mar 2017

Keywords

  • dental pulp cells
  • endodontic regeneration
  • fibrin scaffold
  • platelet rich plasma
  • revitalization

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

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title = "Platelet rich plasma and dentine effect on sheep dental pulp cells regeneration/revitalization ability (in vitro)",
abstract = "Background: Platelet rich plasma (PRP) has been proposed as a scaffold for pulp regeneration/revitalization instead of a blood clot. The aim of the following in vitro study was to evaluate the effect of PRP scaffold on proliferation, migration and differentiation of cultured ovine (sheep) dental pulp cells (ODPC) in the presence of dentine. Methods: PRP was prepared by centrifuging blood at 140 g for 12 min. ODPC were cultured on PRP or platelet poor plasma (PPP) scaffolds with and without dentine discs. Cell proliferation, migration and differentiation rates were assessed. Results: ODPC cultured on PRP scaffold showed significantly greater proliferation rates, migration and mineralization compared with cells on PPP or without a scaffold. Dentine discs reduced the proliferation and mineralization potential of the cells. Conclusions: A PRP scaffold has a positive effect on the proliferation, migration and differentiation of ODPC; however, dentine discs have an adverse effect on the activity of ODPC.",
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Platelet rich plasma and dentine effect on sheep dental pulp cells regeneration/revitalization ability (in vitro). / Altaii, M.; Kaidonis, X.; Koblar, S.; Cathro, P.; Richards, L.

In: Australian Dental Journal, Vol. 62, No. 1, 01.03.2017, p. 39-46.

Research output: Contribution to journalArticle

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AU - Altaii, M.

AU - Kaidonis, X.

AU - Koblar, S.

AU - Cathro, P.

AU - Richards, L.

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AB - Background: Platelet rich plasma (PRP) has been proposed as a scaffold for pulp regeneration/revitalization instead of a blood clot. The aim of the following in vitro study was to evaluate the effect of PRP scaffold on proliferation, migration and differentiation of cultured ovine (sheep) dental pulp cells (ODPC) in the presence of dentine. Methods: PRP was prepared by centrifuging blood at 140 g for 12 min. ODPC were cultured on PRP or platelet poor plasma (PPP) scaffolds with and without dentine discs. Cell proliferation, migration and differentiation rates were assessed. Results: ODPC cultured on PRP scaffold showed significantly greater proliferation rates, migration and mineralization compared with cells on PPP or without a scaffold. Dentine discs reduced the proliferation and mineralization potential of the cells. Conclusions: A PRP scaffold has a positive effect on the proliferation, migration and differentiation of ODPC; however, dentine discs have an adverse effect on the activity of ODPC.

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