Plasma activated coating immobilizes apolipoprotein A-I to stainless steel surfaces in its bioactive form and enhances biocompatibility

Laura Z. Vanags, Joanne T.M. Tan, Miguel Santos, Praveesuda S. Michael, Ziad Ali, Marcela M.M. Bilek, Steven G. Wise, Christina A. Bursill

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We utilized a plasma activated coating (PAC) to covalently bind the active component of high density lipoproteins (HDL), apolipoprotein (apo) A-I, to stainless steel (SS) surfaces. ApoA-I suppresses restenosis and thrombosis and may therefore improve SS stent biocompatibility. PAC-coated SS significantly increased the covalent attachment of apoA-I, compared to SS alone. In static and dynamic flow thrombosis assays, PAC + apoA-I inhibited thrombosis and reduced platelet activation marker p-selectin. PAC + apoA-I reduced smooth muscle cell attachment and proliferation, and augmented EC attachment to PAC. We then coated PAC onto murine SS stents and found it did not peel or delaminate following crimping/expansion. ApoA-I was immobilized onto PAC-SS stents and was retained as a monolayer when exposed to pulsatile flow in vivo in a murine stent model. In conclusion, ApoA-I immobilized on PAC withstands pulsatile flow in vivo and retains its bioactivity, exhibiting anti-thrombotic and anti-restenotic properties, demonstrating the potential to improve stent biocompatibility.

Original languageEnglish
Pages (from-to)2141-2150
Number of pages10
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume13
Issue number7
DOIs
Publication statusPublished - Oct 2017
Externally publishedYes

Keywords

  • Apolipoprotein A-I
  • Biocompatibility
  • Plasma deposition
  • Stent
  • Thrombosis

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

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