Plant-derived MINA-05 inhibits human prostate cancer proliferation in vitro and lymph node spread in vivo

Kate Vandyke, Melanie Y. White, Terry Nguyen-Khuong, Kim Ow, Sharon C.W. Luk, Elizabeth A. Kingsley, Alexandra Rowe, Shiu Fun Pang, Bradley J. Walsh, Pamela J. Russell

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Few treatment options exist for metastatic prostate cancer (PC) that becomes hormone refractory (HRPC). In vitro, plant-derived MINA-05 caused dose-dependent decreases in cell numbers in HRPC cell lines LNCaPC4-2B and PC-3, and in androgen-sensitive LNCaP-FGC, DuCaP, and LAPC-4, by WST-1 assay. MINA-05 pretreatment significantly decreased clonogenic survival in agar and on plastic at 1 x and 2 x IC50 for PC-3 (P < .05 and P < .001, respectively), and at 1/2 x, 1 x, and 2 x IC50 for LNCaP-FGC cells (P < .001). MINA-05 also induced G2M arrest of LNCaP-FGC and PC-3 cells (by flow cytometry) and caused some apoptosis in LNCaP-FGC (sub-G 1 peak on flow, expression of activated caspase-3) but not in PC-3 cells. Western blotting indicated that these cell cycle changes were associated with decreased levels of regulatory proteins cyclin B1 and cdc25C. MINA-05 given daily by gavage for 39 days did not diminish primary orthotopic PC-3 growth in nude mice, but decreased the extent of lymph node invasion at higher doses. We conclude that MINA-05 induces G2M arrest, inhibits cell growth, reduces PC cell regrowth in vitro, and reduces lymph node invasion after orthotopic PC-3 cell implantation in vivo. It has potential as an adjuvant treatment for patients with PC.

Original languageEnglish
Pages (from-to)322-331
Number of pages10
JournalNeoplasia
Volume9
Issue number4
DOIs
Publication statusPublished - 1 Jan 2007
Externally publishedYes

Keywords

  • Clonogenic assays
  • GM block
  • Lymph node metastases
  • MINA-05 herbal treatment
  • Prostate cancer

ASJC Scopus subject areas

  • Cancer Research

Cite this

Vandyke, K., White, M. Y., Nguyen-Khuong, T., Ow, K., Luk, S. C. W., Kingsley, E. A., ... Russell, P. J. (2007). Plant-derived MINA-05 inhibits human prostate cancer proliferation in vitro and lymph node spread in vivo. Neoplasia, 9(4), 322-331. https://doi.org/10.1593/neo.06775