We have examined the phosphorylation of the α-subunit of initiation factor-2 (eIF-2α) in reticulocyte lysates in which translational shut-off was induced by haem-deficiency or by double-stranded RNA. To maximise the phosphorylation of eIF-2α, lysates were supplemented with the broad spectrum phosphatase inhibitor microcystin. Under all conditions tested, serine-51 was the only residue to become labelled. This is consistent with the observation of only two species of eIF-2α in isoelectric focusing/immunoblotting analyses of lysates treated as described above.
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - 15 May 1991|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology