PCR amplification and sequence analysis of GC-rich sequences: Aristaless-related homeobox example

May H. Tan; Jozef Gécz; Cheryl Shoubridge

doi:10.1007/978-1-62703-438-8_8

PCR amplification and sequence analysis of GC-rich sequences: Aristaless-related homeobox example

May H. Tan, Jozef Gécz, Cheryl Shoubridge

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

4 Citations (Scopus)

Abstract

PCR amplification (followed by mutation scanning or direct sequencing) is a technique widely used in mutation detection and molecular studies of disease-causing genes, such as ARX. PCR amplification of high GC-rich regions encounters difficulties using conventional PCR procedures. Here, we present the strategies to amplify and sequence these GC-rich regions for the purposes of mutation screening and other molecular analyses.

Original language	English
Title of host publication	Tandem Repeats in Genes, Proteins, and Disease
Subtitle of host publication	Methods and Protocols
Publisher	Humana Press Inc.
Pages	105-120
Number of pages	16
ISBN (Print)	9781627034371
DOIs	https://doi.org/10.1007/978-1-62703-438-8_8
Publication status	Published - 2013
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1017
ISSN (Print)	1064-3745

Keywords

ARX
GC-rich
PCR
Polyalanine tracts
Sequencing

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-62703-438-8_8

Link to publication in Scopus

Cite this

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abstract = "PCR amplification (followed by mutation scanning or direct sequencing) is a technique widely used in mutation detection and molecular studies of disease-causing genes, such as ARX. PCR amplification of high GC-rich regions encounters difficulties using conventional PCR procedures. Here, we present the strategies to amplify and sequence these GC-rich regions for the purposes of mutation screening and other molecular analyses.",

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PCR amplification and sequence analysis of GC-rich sequences : Aristaless-related homeobox example. / Tan, May H.; Gécz, Jozef; Shoubridge, Cheryl.

Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols. Humana Press Inc., 2013. p. 105-120 (Methods in Molecular Biology; Vol. 1017).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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T2 - Aristaless-related homeobox example

AU - Tan, May H.

AU - Gécz, Jozef

AU - Shoubridge, Cheryl

PY - 2013

Y1 - 2013

N2 - PCR amplification (followed by mutation scanning or direct sequencing) is a technique widely used in mutation detection and molecular studies of disease-causing genes, such as ARX. PCR amplification of high GC-rich regions encounters difficulties using conventional PCR procedures. Here, we present the strategies to amplify and sequence these GC-rich regions for the purposes of mutation screening and other molecular analyses.

AB - PCR amplification (followed by mutation scanning or direct sequencing) is a technique widely used in mutation detection and molecular studies of disease-causing genes, such as ARX. PCR amplification of high GC-rich regions encounters difficulties using conventional PCR procedures. Here, we present the strategies to amplify and sequence these GC-rich regions for the purposes of mutation screening and other molecular analyses.

KW - ARX

KW - GC-rich

KW - PCR

KW - Polyalanine tracts

KW - Sequencing

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SN - 9781627034371

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