PCR amplification and sequence analysis of GC-rich sequences: Aristaless-related homeobox example

May H. Tan, Jozef Gécz, Cheryl Shoubridge

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

4 Citations (Scopus)

Abstract

PCR amplification (followed by mutation scanning or direct sequencing) is a technique widely used in mutation detection and molecular studies of disease-causing genes, such as ARX. PCR amplification of high GC-rich regions encounters difficulties using conventional PCR procedures. Here, we present the strategies to amplify and sequence these GC-rich regions for the purposes of mutation screening and other molecular analyses.

Original languageEnglish
Title of host publicationTandem Repeats in Genes, Proteins, and Disease
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages105-120
Number of pages16
ISBN (Print)9781627034371
DOIs
Publication statusPublished - 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1017
ISSN (Print)1064-3745

Keywords

  • ARX
  • GC-rich
  • PCR
  • Polyalanine tracts
  • Sequencing

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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